53
54
52
51
34
2
36
2
71
22
23
2
28
15
39
4
42
20
29
2
25 21
38
2
14
33
13
30
19
31
PRUD
PUNI
FOZI
TSAM
15
64
BLUM
SJOS
05
45
APIA
GUAR
46
17
37
02
35
60
2
ICOM
2
61
49
27
03
62
50
GUAP
04
01
07
63
48
SSEB
IBEL
18
16
IGRA
06
43
10
69
PASQ
ANGR
09
44
66
24
73
08
11
40
2
68
55 26
58
41
32
67
59
57
2
RESE
72
70
TERE
12
55
47
65
Figure S1. MtDNA haplotype network from 17 populations of Tetragonisca angustula. Each colour refers to one population. The 73 haplotypes are
denominated by black numbers. Line between haplotypes indicates one nucleotide substitution and red numbers indicate the number of nucleotide substitutions
greater than one. Population abbreviations as in Table 1.
Table S1. Information about sampling sites. RJ: Rio de Janeiro. MG: Minas Gerais. SP: São Paulo. PR: Paraná. SC: Santa Catarina. Geographic coordinates are in degrees
(D), minutes (M) and seconds (S). N: number of sampled Tetragonisca angustula bees. (n): indicates bees collected in nests.
Coordinates
Population
City
State
Site code
South
West
N
D
M
S
D
M
S
PETRO 1
Teresópolis
Petrópolis
RJ
22
23
17.3
43
7
57.3
1
PETRO 2
Teresópolis
Petrópolis
RJ
22
25
9.4
43
10
25.8
1
PETRO 3
Teresópolis
Petrópolis
RJ
22
25
21.2
43
7
59.1
1
PETRO 4
Teresópolis
Petrópolis
RJ
22
27
54.4
43
9
32.5
1
PETRO 5
Teresópolis
Petrópolis
RJ
22
30
54.7
43
10
9.6
1
TERE 1
Teresópolis
Teresópolis
RJ
23
24
28.3
42
57
53.2
1
TERE 10
Teresópolis
Teresópolis
RJ
22
26
28.1
42
59
17.7
2
TERE 11
Teresópolis
Teresópolis
RJ
22
26
27.6
42
59
6.8
1 (n)
TERE 12
Teresópolis
Teresópolis
RJ
22
26
31.2
42
59
7.1
2
TERE 13
Teresópolis
Teresópolis
RJ
22
26
31
42
58
55.2
2
TERE 14
Teresópolis
Teresópolis
RJ
22
26
44.6
42
58
58.7
2
TERE 15
Teresópolis
Teresópolis
RJ
22
26
44.6
42
58
37.4
1
TERE 16
Teresópolis
Teresópolis
RJ
22
26
43
42
58
28.5
2
TERE 17
Teresópolis
Teresópolis
RJ
22
26
35.4
42
58
38.1
2
TERE 18
Teresópolis
Teresópolis
RJ
22
27
8.2
42
58
4.1
2
TERE 19
Teresópolis
Teresópolis
RJ
22
27
8.7
42
57
53.8
2
TERE 2
Teresópolis
Teresópolis
RJ
22
26
58.4
42
58
40.4
1
TERE 20
Teresópolis
Teresópolis
RJ
22
27
0.1
42
58
25.7
2
TERE 21
Teresópolis
Teresópolis
RJ
22
25
43.6
42
58
56.3
2
TERE 22
Teresópolis
Teresópolis
RJ
22
25
47
42
59
0
2
TERE 23
Teresópolis
Teresópolis
RJ
22
25
37.1
42
59
0.6
2
TERE 24
Teresópolis
Teresópolis
RJ
22
25
36.6
42
59
15
2
TERE 25
Teresópolis
Teresópolis
RJ
22
25
24.9
42
59
26.5
2
TERE 26
Teresópolis
Teresópolis
RJ
22
25
11
42
59
48
2
TERE 27
Teresópolis
Teresópolis
RJ
22
25
4.1
42
59
58.8
1
TERE 28
Teresópolis
Teresópolis
RJ
22
25
28.1
42
59
17.5
3
TERE 5
Teresópolis
Teresópolis
RJ
22
26
6.5
42
58
33.9
1
TERE 6
Teresópolis
Teresópolis
RJ
22
25
27.5
42
57
25.2
1
TERE 7
Teresópolis
Teresópolis
RJ
22
25
14.2
42
59
2.2
1
TERE 8
Teresópolis
Teresópolis
RJ
22
26
23.1
42
59
34
2
TERE 9
Teresópolis
Teresópolis
RJ
22
26
28.4
42
59
37.7
2
MAUÁ 1
Resende
Visconde de Mauá
RJ
22
19
55.3
44
32
22.1
1
PENE 1
Resende
Resende
RJ
22
27
24.3
44
30
47.9
2
PENE 10
Resende
Resende
RJ
22
27
36.3
44
30
46.5
2
PENE 11
Resende
Resende
RJ
22
27
33.7
44
30
56.3
2
PENE 12
Resende
Resende
RJ
22
27
27.8
44
30
54.9
2
PENE 13
Resende
Resende
RJ
22
27
12.1
44
30
51.9
1
PENE 14
Resende
Resende
RJ
22
27
10.2
44
31
3.2
2
PENE 15
Resende
Resende
RJ
22
27
2.1
44
31
17.8
2
PENE 16
Resende
Resende
RJ
22
26
46.4
44
31
33.8
2
PENE 17
Resende
Resende
RJ
22
26
58
44
31
39.4
2
PENE 18
Resende
Resende
RJ
22
26
13
44
31
50.5
2
PENE 19
Resende
Resende
RJ
22
25
12.4
44
32
38.4
2
PENE 2
Resende
Resende
RJ
22
27
23.9
44
30
56.2
2
PENE 20
Resende
Resende
RJ
22
25
9.8
44
32
49.9
2
PENE 21
Resende
Resende
RJ
22
25
11.3
44
32
30.6
2
PENE 22
Resende
Resende
RJ
22
25
14
44
32
16
2
PENE 23
Resende
Resende
RJ
22
25
41.8
44
31
55.2
1
PENE 24
Resende
Resende
RJ
22
26
47.3
44
31
51.6
2
PENE 25
Resende
Resende
RJ
22
26
36.2
44
31
43.2
2
PENE 26
Resende
Resende
RJ
22
25
53.1
44
32
5.4
2
PENE 28
Resende
Resende
RJ
22
27
17.4
44
30
53.8
1 (n)
PENE 3
Resende
Resende
RJ
22
27
27.1
44
30
40.3
2
PENE 4
Resende
Resende
RJ
22
27
17.2
44
31
9.5
2
PENE 5
Resende
Resende
RJ
22
27
13.8
44
31
17.1
2
PENE 6
Resende
Resende
RJ
22
26
57.1
44
31
27.4
2
PENE 7
PENE 8
PENE 9
ITAMO 1
ITAMO 10
ITAMO 11
ITAMO 12
ITAMO 13
ITAMO 3
ITAMO 4
ITAMO 5
ITAMO 6
ITAMO 7
ITAMO 9
ITANH 1
ITANH 2
ITANH 3
PASSA 1
PASSA 4
PASSA 5
PASSA 6
PASSA 7
POUSOA 1
POUSOA 2
SLOU 1
SSRV 1
SSRV 2
ANGRA 1
ANGRA 10
ANGRA 11
ANGRA 12
ANGRA 13
ANGRA 14
ANGRA 15
ANGRA 16
ANGRA 17
ANGRA 19
ANGRA 20
ANGRA 21
ANGRA 22
ANGRA 23
ANGRA 24
ANGRA 25
ANGRA 26
ANGRA 27
ANGRA 28
ANGRA 3
ANGRA 4
ANGRA 5
ANGRA 6
ANGRA 7
ANGRA 8
ANGRA 9
PAR 10
PAR 11
PAR 14
PAR 3
PAR 4
PAR 9
IGRA 11
IGRA 13
Resende
Resende
Resende
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Ilha Grande
Ilha Grande
Resende
Resende
Resende
Itamonte
Itamonte
Itamonte
Itamonte
Itamonte
Itamonte
Itamonte
Itamonte
Itamonte
Itamonte
Itamonte
Itanhandu
Itanhandu
Itanhandu
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Passa Quatro
Pouso Alto
Pouso Alto
São Lourenço
São Sebastião do Rio Verde
São Sebastião do Rio Verde
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Parati
Parati
Parati
Angra dos Reis
Angra dos Reis
RJ
RJ
RJ
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
MG
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
23
23
22
22
22
23
23
23
23
23
23
23
23
22
22
22
22
22
22
23
23
23
22
22
23
23
23
23
22
23
23
23
23
23
26
26
27
17
16
16
18
15
16
15
18
18
17
17
17
17
17
22
23
23
22
22
11
12
7
12
13
0
0
59
59
59
0
0
1
0
1
0
1
1
59
59
59
58
59
58
0
0
3
59
59
0
0
0
0
57
13
13
2
7
6
46.8
34.4
33.3
37.9
59.5
53.6
3.9
8.7
18.6
56.4
50.4
5.5
44.2
20.8
32.7
22
52.8
58.8
45.8
23.1
58.6
38.8
49.1
3.7
23.7
58.8
13
19
3.7
22.4
19.4
24.7
6.5
39.3
21.3
44.4
53.5
43
13.3
21.2
31.7
32.8
1.5
57.4
6.9
18.5
9.8
48
0.4
57.1
59.1
11.9
8.9
51
42.9
52.9
29.9
28.3
41.5
46.8
38.1
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
45
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
31
31
30
51
52
51
52
55
52
54
51
51
51
51
56
56
56
58
58
57
58
57
58
58
2
58
58
18
14
14
14
14
13
13
13
13
9
19
20
20
20
19
18
18
18
18
16
13
14
14
14
14
14
32
31
26
43
45
35
11
12
27.6
14
36.1
48.2
15.3
49.6
26.8
55.5
35.1
10.7
15.8
36.4
46.9
46.2
13.9
8.4
20.4
1
7
54.9
19.7
53.5
21.6
33.6
22.9
38.4
38.4
37.8
16.8
2.4
7.8
16.5
51.2
19.4
38
13.8
56.7
19.6
0.1
34
15.3
31.7
56.2
36.4
25.3
3.8
14.2
14.8
41.1
20.4
25
17.8
12.4
1.9
43.2
16.8
46.3
4.9
42.3
0.7
57.7
2
1
2
3 (n)
2
2
2
1
2
2
3
3
2
2
3
2
2
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
2
2
2
2
2
2
2
2
2
3
2
2
2
2
2
2
2
2
2
2
2
2
1 (n)
9 (n)
IGRA 14
IGRA 15
IGRA 16
IGRA 17
IGRA 2
IGRA 20
IGRA 21
IGRA 23
IGRA 28
IGRA 29
IGRA 30
IGRA 31
IGRA 32
IGRA 33
IGRA 34
IGRA 36
IGRA 38
IGRA 39
IGRA 40
IGRA 42
IGRA 51
IGRA 57
IGRA 6
IGRA 8
IGRA 9
SSEB 1
SSEB 10
SSEB 13
SSEB 14
SSEB 15
SSEB 16
SSEB 19
SSEB 2
SSEB 20
SSEB 21
SSEB 22
SSEB 23
SSEB 24
SSEB 25
SSEB 27
SSEB 3
SSEB 30
SSEB 31
SSEB 32
SSEB 33
SSEB 34
SSEB 4
SSEB 41
SSEB 5
SSEB 7
SSEB 8
SSEB 9
IBELA 12
IBELA 13
IBELA 16
IBELA 17
IBELA 18
IBELA 19
IBELA 20
IBELA 21
IBELA 24
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
Ilha Grande
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
Angra dos Reis
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
São Sebastião
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
RJ
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
9
9
8
8
8
9
10
10
8
8
9
9
8
8
8
5
8
8
7
9
7
9
10
10
8
49
50
43
43
48
47
47
49
46
46
46
45
45
48
48
45
49
49
49
48
48
46
46
47
48
49
49
44
44
45
48
49
51
51
54
55
21.5
17.5
33
19.6
40.6
22.4
23.9
51.2
22.1
32
30.9
49.6
31
42.8
37.8
10.2
9.1
8.5
57.2
17.3
1.8
53.9
48.1
51.2
2.3
6.4
1.7
49
21.7
0.5
40.1
25.3
3
50.7
43.6
4
56.5
25.7
12.3
33.9
29.9
21.6
39.6
19.8
59.9
59.8
19.8
18.8
14.6
3
19.3
56.1
19.5
6
13.2
56
1.7
1.1
43.3
39.9
32.8
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
44
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
19
19
18
18
10
20
20
20
8
8
8
8
9
10
9
14
9
9
10
11
15
20
11
11
10
28
30
24
25
24
24
24
27
23
24
24
24
24
24
24
24
26
25
27
32
31
24
42
23
24
26
30
17
18
20
22
21
24
25
27
26
54.2
33.2
52.4
44.5
14.2
8.6
24.4
46.3
59.5
26.1
24.7
22.8
41.2
2.9
56.6
1.1
14.1
16.5
9.9
47.3
32.5
55.2
21
21.4
25.7
3.3
37.5
4.8
31.7
18.3
4.6
2.8
45.6
54.6
11.3
24.9
25.8
48.2
21.8
44.3
48.8
21.3
50.3
17.2
3.1
56.8
17.2
4.3
55
19.6
2.4
26
45.1
29.5
47.9
2.1
52.9
41.7
29
27.4
55.1
1 (n)
1 (n)
1 (n)
3
16 (n)
1 (n)
2
1 (n)
1
1
1 (n)
1
1 (n)
1 (n)
1 (n)
2
1 (n)
1 (n)
1 (n)
3
2
2
7 (n)
1 (n)
1 (n)
1
1 (n)
3
3
2
1 (n)
2
1 (n)
2
2
2
2
3
2
2
3
1
1
1
2
1 (n)
3
1 (n)
3
3
2
1 (n)
1
1
1
1 (n)
2
2
2
1
2
IBELA 25
IBELA 27
IBELA 28
IBELA 29
IBELA 3
IBELA 30
IBELA 31
IBELA 32
IBELA 33
IBELA 34
IBELA 35
IBELA 36
IBELA 37
IBELA 38
IBELA 39
IBELA 4
IBELA 40
IBELA 44
IBELA 45
IBELA 46
IBELA 47
IBELA 48
IBELA 49
IBELA 53
IBELA 56
IBELA 57
IBELA 58
IGUA 1
IGUA 10
IGUA 11
IGUA 12
IGUA 13
IGUA 14
IGUA 15
IGUA 16
IGUA 17
IGUA 18
IGUA 19
IGUA 2
IGUA 20
IGUA 21
IGUA 22
IGUA 24
IGUA 25
IGUA 26
IGUA 27
IGUA 3
IGUA 4
IGUA 5
IGUA 6
IGUA 7
IGUA 8
IGUA 9
ICOMP 1
ICOMP 11
ICOMP 12
ICOMP 13
ICOMP 14
ICOMP 15
ICOMP 16
ICOMP 17
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Ilha de São Sebastião
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Ilhabela
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Iguape
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
23
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
53
52
51
51
56
49
46
45
45
43
44
43
47
49
51
56
51
49
49
48
48
47
50
46
47
50
51
42
42
42
41
41
42
41
41
40
38
38
42
41
42
42
42
41
41
40
42
42
42
42
41
40
40
44
44
43
43
44
44
44
44
31
24.8
44.9
11.6
10
29.3
43.7
34.6
36
58.9
3
59.7
14.9
28.1
1.9
9
35.1
19.6
25.1
22.7
13.3
27.7
48
32.6
31.3
47.7
6.5
57.6
26
8.8
44.1
47.3
11.7
10.9
9.2
33.4
26.1
20.1
48.7
47.2
25.6
35.3
22.6
56.6
34
58.7
23
45.5
38.4
16.9
8.6
54.4
46.6
27.8
3.2
36
40.5
12.2
21.2
31.3
35.1
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
47
27
26
25
24
25
22
21
21
20
20
18
18
21
21
19
25
17
21
22
21
21
21
24
21
21
24
24
34
33
33
33
33
33
29
28
28
29
30
34
33
33
34
33
33
31
27
34
33
33
33
27
27
27
32
31
32
32
32
32
32
33
20.6
26
39.6
43.9
41
35.2
28
2
25.7
30.4
38
6.7
30.2
11.1
4.5
41
17.6
43.2
6.9
50.6
41.8
47.1
20.9
13.4
21.6
21
39.2
27.3
20.7
27.3
50.3
38.1
51.4
49.4
2.9
16.5
18.4
0.4
26.6
55.1
4.7
27.4
39.8
42.8
48.9
43.9
8.3
31.8
20.4
11.8
45.4
7
45.4
45.9
35.9
11.9
22.4
24.5
35.1
53.4
13.7
1
1
2
1
2
2
1
1
1
1
1
1
1
1
2
1 (n)
1 (n)
3
2
2
2
2
2
1
1
1
1
2
2
2
2
2
2
1 (n)
2
1 (n)
2
2
2
2
2
2
2
2
2
2
1
3
2
2
2
2
2
1
4
3
3
3
3
3
3
ICOMP 18
ICOMP 19
ICOMP 20
ICOMP 21
ICOMP 23
ICOMP 24
ICOMP 25
ICOMP 26
ICOMP 27
ICOMP 28
ICOMP 7
ICARD 13
APIAÍ 1
APIAÍ 18
APIAÍ 19
APIAÍ 22
APIAÍ 4
APIAÍ 7
APIAÍ 8
APIAÍ 9
ARAÇAÍBA
IPOR 11
IPOR 12
IPOR 14
IPOR 15
IPOR 18
IPOR 19
IPOR 20
IPOR 22
IPOR 4
ITAOCA 1
ITAOCA 2
ITAOCA 3
ITAOCA 4
GUARATUBA 12
GUARATUBA 14
GUARATUBA 15
GUARATUBA 16
GUARATUBA 17
GUARATUBA 18
GUARATUBA 19
GUARATUBA 2
GUARATUBA 21
GUARATUBA 22
GUARATUBA 23
GUARATUBA 28
GUARATUBA 30
GUARATUBA 31
GUARATUBA 32
GUARATUBA 33
GUARATUBA 34
GUARATUBA 35
GUARATUBA 37
GUARATUBA 38
GUARATUBA 39
GUARATUBA 6
GUARATUBA 8
GUARATUBA 9
BLUME 1
BLUME 10
BLUME 11
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha do Cardoso
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Blumenau
Blumenau
Blumenau
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Ilha Comprida
Cananéia
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Apiaí
Iporanga
Iporanga
Iporanga
Iporanga
Iporanga
Iporanga
Iporanga
Iporanga
Iporanga
Itaóca
Itaóca
Itaóca
Itaóca
Itapoá
Itapoá
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Guaratuba
Garuva
Blumenau
Blumenau
Blumenau
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SC
SC
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
SC
SC
SC
SC
24
24
24
24
24
24
24
24
24
24
24
25
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
26
26
25
25
25
25
25
25
25
25
25
26
26
26
26
26
26
26
26
26
26
25
25
25
26
26
26
44
45
43
42
45
44
42
43
44
44
42
5
30
32
32
29
30
30
30
30
25
35
34
35
35
35
35
36
33
35
36
37
38
38
7
3
56
58
52
52
52
52
52
53
53
1
1
2
2
3
4
4
6
7
8
52
55
59
53
56
54
35.6
10.4
47.7
41.8
7.2
30.6
39.5
31
3.7
33.9
30.7
36
28
26.3
22.4
33.7
59
32
57
20
42
7.2
54.7
14.7
26.6
9.3
10.9
31.4
19.3
11.3
9.8
50.4
26.9
40.3
3.4
22.5
56.5
18.8
28.2
31.1
44.4
43
32.9
4.2
42.3
24.4
43.8
25.1
55.6
23.8
0.3
33.6
11.1
20.6
2.1
55
44.6
5.7
7.3
31
19
47
47
47
47
47
47
47
47
47
47
47
47
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
49
49
49
33
33
33
29
33
32
29
32
31
32
29
55
50
49
48
50
50
50
51
50
51
35
35
35
35
35
35
37
40
35
52
51
50
50
36
36
35
41
34
34
34
34
34
34
34
36
36
36
36
36
37
36
36
36
35
34
43
42
10
4
4
20.4
41.6
27.8
9.6
36.8
29.5
21.7
7.3
48.9
33.4
15
45.9
23
15.6
28.2
44.5
31
39
12
33
21
37.3
30.8
48.7
52.3
30.2
42.5
24.6
43.2
40.1
29.2
18.4
29.7
6.8
12.2
54.6
46.8
11.4
38.6
23.7
8.6
23
49.9
16.6
9.2
36.9
48.4
48.3
55.9
58
3.2
20
13.8
17.4
55.9
29.2
43.3
44.1
12.9
13.3
44.1
3
3
3
3
1 (n)
2
3
2
3
1
3
1 (n)
1 (n)
3
2 (n)
2
3
2
2
1
1 (n)
3
3
3
3
3
3
3
3
1
3
3
2
2
2
1
1
1
2
2
2
1
2
2
2
3
3
2
4
2
3
2
2
2
3
1 (n)
4 (n)
1
2
1 (n)
1 (n)
BLUME 12
BLUME 13
BLUME 14
BLUME 15
BLUME 17
BLUME 18
BLUME 19
BLUME 2
BLUME 20
BLUME 22
BLUME 23
BLUME 25
BLUME 26
BLUME 27
BLUME 28
BLUME 29
BLUME 3
BLUME 30
BLUME 31
BLUME 4
BLUME 5
BLUME 6
BLUME 8
BLUME 9
BIGU 1
BIGU 12
BIGU 13
BIGU 14
BIGU 2
BIGU 3
BIGU 6
BIGU 7
PALHO 1
PALHO 2
PALHO 6
PALHO 9
SJOS 10
SJOS 14
SJOS 5
SJOS 7
SJOS 8
SJOS 9
SJOZ 1
SJOZ 2
SJOZ 3
TIJU 1
TIJU 3
FLORI 40
FLORI 41
FLORI 42
FLORI 43
FLORI 44
FLORI 46
FLORI 47
FLORI 48
FLORI 49
FLORI 50
FLORI 51
FLORI 52
FLORI 53
FLORI 54
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
São José
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Indaial
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Blumenau
Indaial
Blumenau
Blumenau
Indaial
Indaial
Indaial
Ascurra
Blumenau
Biguaçu
Biguaçu
Biguaçu
Biguaçu
Biguaçu
Biguaçu
Governador Celso Ramos
Governador Celso Ramos
Palhoça
Palhoça
Palhoça
Palhoça
São José
São José
São José
São José
São José
São José
São José
São José
São José
Tijucas
Tijucas
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
27
55
55
54
54
54
54
53
53
53
52
55
57
57
58
59
59
53
52
52
54
55
57
58
55
28
31
31
30
26
23
23
22
38
37
40
42
35
35
33
32
32
35
36
37
38
14
14
36
36
35
36
36
30
35
36
30
29
30
32
35
35
36.5
9.4
5.6
46.7
44.8
23
38.5
23.1
21.1
44
33.7
4.6
31.7
22.7
15.4
39.4
21.3
33.1
14.7
9.4
39.6
1.1
1.9
11.7
14.1
32.3
21
30.1
52
58
54.9
40.3
23.1
0
31.5
53.6
45.7
8.7
53.1
42.9
12.6
16.6
47.5
28.6
21.9
35.3
25.4
2.4
12.1
51.2
21.4
19.4
41
30.2
54.9
24.8
59.3
18.8
18.1
28.9
15.9
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
49
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
48
3
3
2
3
3
4
3
11
4
4
3
4
4
4
4
6
13
4
5
14
16
17
21
3
38
38
39
39
37
36
33
33
40
41
40
42
37
43
38
38
38
37
37
37
38
37
38
27
28
27
28
28
29
29
29
31
31
31
30
31
31
24.9
45
57.6
35.8
53.8
0.6
29.1
37.1
48.9
47.7
56.3
30.7
35.2
34.5
50.2
5.1
25.3
1.4
25.8
45.2
40.6
11
48.8
53.7
25.6
48.5
23
27.2
50.3
10.3
52.3
37.5
10.9
23.9
48.2
22.5
22.8
6.2
5
25.6
47.8
9.6
45
54.1
5
34.3
27.7
52.2
5.8
52.7
5.9
25.7
8
45.6
4.5
11
26.2
1.4
49.1
32.2
31.9
2
2
2
2
1
2
2
2
2
2
4
2
2
2
2
2
2
1
1
2
2
2
2
1 (n)
3
3
4
2
3
3
2
2
2
2
2
2
2
2
3
3
3
3
2
2
2
2
2
6
6
6
6
3
3
4
6
5
5
5
2
1
2
FLORI 55
FLORI 56
FLORI 57
FLORI 58
FLORI 59
GUAMIR 1
IMBIT 10
IMBIT 2
IMBIT 3
IMBIT 4
IMBIT 5
IMBIT 6
IMBIT 7
IMBIT 9
IRATI 3
IRATI 4
PRUDE 10
PRUDE 11
PRUDE 14
PRUDE 15
PRUDE 2
PRUDE 3
PRUDE 4
PRUDE 5
PRUDE 7
PRUDE 8
PRUDE 9
CAÇA 1
CAÇA 2
CAÇA 3
CAÇA 4
CAÇA 5
CAÇA 6
CAÇA 7
CAÇA 8
GCAR 1
GCAR 2
GCAR 3
UNVI 1
UNVI 10
UNVI 11
UNVI 2
UNVI 3
UNVI 4
UNVI 5
UNVI 7
UNVI 9
CAPA 1
CAZU 1
CAZU 2
CAZU 3
CLMA 1
FOZ 1
FOZ 2
FOZ 3
LIND 1
MATE 1
MEDI 1
SIGU 1
SLUC 1
SMAR 1
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Ilha de Santa Catarina
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Porto União
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Florianópolis
Guamiranga
Imbituva
Imbituva
Imbituva
Imbituva
Imbituva
Imbituva
Imbituva
Imbituva
Irati
Irati
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Guamiranga
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Prudentópolis
Caçador
Caçador
Caçador
Caçador
Caçador
Caçador
Caçador
Caçador
General Carneiro
General Carneiro
General Carneiro
Porto União
União da Vitória
União da Vitória
Porto União
União da Vitória
União da Vitória
União da Vitória
União da Vitória
União da Vitória
Capanema
Céu Azul
Céu Azul
Céu Azul
Capitão Leônidas Marques
Foz do Iguaçu
Foz do Iguaçu
Foz do Iguaçu
Lindoeste
Matelândia
Medianeira
Serranópolis do Iguaçu
Santa Lúcia
Santa Maria
SC
SC
SC
SC
SC
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
SC
SC
SC
SC
SC
SC
SC
SC
PR
PR
PR
SC
PR
PR
SC
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
PR
27
27
27
27
27
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
26
25
25
25
25
25
25
25
25
25
25
25
25
25
25
36
36
36
35
33
11
13
13
13
13
13
14
14
14
28
29
13
12
12
12
11
11
11
12
12
12
12
47
47
47
47
46
46
46
46
25
25
26
14
13
13
15
14
13
12
13
14
39
9
8
9
29
26
35
41
15
14
17
22
24
8
5.9
25.3
30.9
30.7
33.3
41.4
52.5
37.5
40.1
44.3
36.6
2.9
34
23.7
58.7
4.8
12.4
54.9
22.2
20.7
35.6
21.8
57
31.9
50.3
41.8
8.1
4.7
15.9
5.7
22.9
55.2
28.6
26.9
14.6
21.5
43.8
7.8
13.7
58.9
53.7
15.4
15.9
33
39.5
56.6
11.6
42.9
0.1
56.1
29.4
22
54.1
25
30
0.7
29.2
59.2
53.8
26.5
35.3
48
48
48
48
48
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
51
51
51
51
51
51
51
51
51
51
51
51
51
51
51
51
51
51
51
53
53
53
53
53
54
54
54
53
53
54
54
53
53
31
31
30
29
29
48
36
36
36
36
35
35
35
35
38
38
58
58
57
58
48
55
58
58
58
59
59
59
0
0
0
0
0
0
1
18
19
19
4
2
3
5
5
5
5
3
5
48
51
50
51
36
35
35
26
34
59
5
3
34
35
26.9
6.7
57.6
37.1
33.9
50.4
52.2
49.8
29.5
8.4
57.1
43.5
27.9
16
57.6
35.9
59.3
32.2
53.5
56.9
41.1
9.1
14
25.2
32.1
28.8
31.3
53.6
24.9
39.9
48.5
54.9
50.2
20.4
22.6
49.8
6.6
15.2
41.3
52.3
57
20.3
23.5
48.1
9.9
30.1
42.8
30.5
20.6
59.5
23.9
24.5
5.1
25
3.9
44.3
3
40.8
14.4
2.7
11.3
1
2
1
1
1
2
3
3
3
4
3
3
3
3
3
1
9 (n)
2 (n)
2
2
2
2
2
1
2
2
2
3
3
3
3
3
1 (n)
3
3
3
3
3
3
3
3
2
1 (n)
2
1
3
1
3
2 (n)
6 (n)
9 (n)
3
4
3
1
4
3
3
1 (n)
2
2
SMI 1
STO 1
ANHU 1
ANHU 2
MIRP 1
PBER 1
PEPI 1
PEPI 2
PEPI 3
PIRAPZ 1
PIRAPZ 2
PIRAPZ 3
PIRAPZ 4
PLNS 1
PLNS 2
PVEN 1
SANA 2
SANA 4
SANA 5
SANA 6
SAND 1
TARAB 1
TARAB 2
TSAM 1
TSAM 11
TSAM 14
TSAM 15
TSAM 16
TSAM 17
TSAM 18
TSAM 19
TSAM 2
TSAM 5
TSAM 6
TSAM 8
Foz do Iguaçu
Foz do Iguaçu
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
São Miguel do Iguaçu
Santa Tereza do Oeste
Anhumas
Anhumas
Mirante do Paranapanema
Presidente Bernardes
Presidente Epitácio
Presidente Epitácio
Presidente Epitácio
Pirapozinho
Pirapozinho
Pirapozinho
Pirapozinho
Teodoro Sampaio
Teodoro Sampaio
Presidente Venceslau
Santo Anastácio
Santo Anastácio
Santo Anastácio
Santo Anastácio
Sandovalina
Tarabai
Tarabai
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
Teodoro Sampaio
PR
PR
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
SP
25
25
22
22
22
22
21
21
21
22
22
22
22
22
22
21
21
21
21
21
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
21
3
17
17
17
1
45
45
45
16
16
16
16
18
19
53
58
58
58
58
27
18
18
32
34
32
32
31
31
32
31
37
31
31
31
5.5
26.9
53.1
36.3
50.9
10.5
23
29.9
34.9
15.8
34.7
20
53.4
59.8
0.9
23.9
20.6
42.6
38
30.3
14.3
12.1
12.6
12.3
14.8
16.3
0.3
56.7
50.1
18.1
27.7
12
41.5
26.4
4.6
54
53
51
51
51
51
52
52
52
51
51
51
51
52
52
51
51
51
51
51
51
51
51
52
52
52
52
52
52
52
52
52
52
52
52
14
37
23
23
54
32
6
6
6
28
29
29
30
14
14
50
39
39
38
38
45
33
33
10
11
10
10
10
9
9
10
10
10
10
18
27.4
1.2
6.6
27.4
26.8
41.8
45.2
33.5
31.7
39
59.2
42.6
43.8
50.9
33.2
17.4
6.2
25.8
52.4
29
40.6
16.5
58.2
39.1
0.8
49.5
31.7
27
49.4
33.4
19
14.9
47.1
7.9
59.5
5
3
3
3
3
2
2
1
1
3
3
3
3
3
2
3
3
3
3
3
3
3
2
1 (n)
1
1 (n)
4 (n)
3 (n)
2
2
2
7 (n)
3
1
1 (n)
Table S2. Islands visited for Tetragonisca angustula sampling. Their area and distance to the mainland
(DM) are presented.
Island
Ilha Grande
Ilha Anchieta
Ilha do Tamanduá
Ilha de São Sebastião
Ilha de Búzios
Ilha da Vitória
Ilha Monte de Trigo
Ilha Comprida
Ilha do Cardoso
Ilha do Mel
Ilha de Santa Catarina
Area (km2) DM (km)
193.0
2.0
8.3
0.5
1.1
0.5
335.9
1.8
7.5
24.1
2.2
38.0
1.3
10.2
200.0
0.3
225.0
0.1
27.6
2.7
451.0
0.5
Table S3. Loci and populations that showed significant deviation from Hardy-Weinberg equilibrium after
Sequential Goodness of Fit test in Tetragonisca angustula.
Locus
Tang03
Tang11
Tang12
Tang65
Population
São José
São José
Angra dos Reis
Foz do Iguaçu
P-value
0.016
0.003
0.033
0.007
Table S4. Pairwise index of differentiation (θ) from microsatellite data of Tetragonisca angustula (all P = 0.0000). Population abbreviations as in Table 1.
RESE
PASQ
ANGR
IGRA
SSEB
IBEL
GUAP
ICOM
APIA
GUAR
BLUM
SJOS
PRUD
PUNI
FOZI
TSAM
TERE
0.0297
0.1057
0.0484
0.1046
0.0878
0.1510
0.1357
0.1031
0.1352
0.1387
0.1484
0.1413
0.1043
0.2944
0.2783
0.1019
RESE
PASQ
ANGR
IGRA
SSEB
IBEL
GUAP
ICOM
APIA
GUAR
BLUM
SJOS
PRUD
PUNI
FOZI
0.0832
0.0277
0.0802
0.0649
0.1342
0.1085
0.0908
0.1093
0.1177
0.1223
0.1222
0.0920
0.2831
0.2649
0.0878
0.1059
0.1720
0.1674
0.1951
0.1992
0.1546
0.1936
0.1933
0.2018
0.2133
0.0752
0.3773
0.3623
0.0653
0.0600
0.0545
0.1129
0.0894
0.0734
0.0887
0.1098
0.1164
0.1149
0.1183
0.2647
0.2506
0.1212
0.0478
0.1276
0.0792
0.0809
0.0874
0.0998
0.0924
0.1018
0.1823
0.3091
0.2806
0.1722
0.0915
0.0430
0.0643
0.0467
0.0578
0.0630
0.0573
0.1787
0.2797
0.2477
0.1712
0.1025
0.1061
0.0915
0.1079
0.1231
0.1038
0.2249
0.3302
0.3052
0.2089
0.0491
0.0163
0.0504
0.0327
0.0475
0.2095
0.3229
0.2939
0.2027
0.0670
0.0804
0.0837
0.1060
0.1638
0.3387
0.3097
0.1641
0.0257
0.0202
0.0283
0.2163
0.3249
0.2896
0.2078
0.0137
0.0247
0.2040
0.3471
0.3047
0.1981
0.0159
0.2143
0.3466
0.3044
0.2049
0.2272
0.3290
0.2876
0.2155
0.3778
0.3656
0.0188
0.0239
0.3723
0.3598
Table S5. Migration rates and direction between 17 populations of Tetragonisca angustula as estimated by BAYESASS. Estimated values are means of 5
independent runs and are listed for each population pair with their respective 95% confidence intervals (in parentheses). Source populations are in the columns
and destination populations in the rows. Values with 95% CI excluding zero are in bold.
FROM
TO
TERE
RESE
PASQ
ANGR
IGRA
SSEB
IBEL
GUAP
ICOM
TERE
0.8745 (0.8285 - 0.9204)
0.0160 (-0.0093 - 0.0413)
0.0111 (-0.0075 - 0.0298)
0.0152 (-0.0085 - 0.0388)
0.0067 (-0.0061 - 0.0196)
0.0062 (-0.0058 - 0.0183)
0.0057 (-0.0053 - 0.0168)
0.0097 (-0.0052 - 0.0245)
0.0055 (-0.0052 - 0.0161)
RESE
0.0217 (-0.0102 - 0.0537)
0.8070 (0.7169 - 0.8971)
0.0068 (-0.0062 - 0.0199)
0.0579 (-0.0188 - 0.1347)
0.0086 (-0.0071 - 0.0243)
0.0157 (-0.0077 - 0.0390)
0.0140 (-0.0062 - 0.0343)
0.0099 (-0.0080 - 0.0277)
0.0056 (-0.0050 - 0.0161)
PASQ
0.0051 (-0.0049 - 0.0151)
0.0053 (-0.0050 - 0.0156)
0.8955 (0.8483 - 0.9426)
0.0064 (-0.0055 - 0.0183)
0.0053 (-0.0050 - 0.0155)
0.0052 (-0.0049 - 0.0153)
0.0052 (-0.0050 - 0.0154)
0.0067 (-0.0054 - 0.0187)
0.0052 (-0.0047 - 0.0150)
ANGR
0.0067 (-0.0057 - 0.0190)
0.0178 (-0.0136 - 0.0492)
0.0057 (-0.0051 - 0.0166)
0.8691 (0.8181 - 0.9201)
0.0067 (-0.0057 - 0.0191)
0.0323 (0.0007 - 0.0639)
0.0057 (-0.0053 - 0.0167)
0.0078 (-0.0062 - 0.0218)
0.0045 (-0.0043 - 0.0133)
IGRA
0.0072 (-0.0044 - 0.0189)
0.0047 (-0.0043 - 0.0136)
0.0047 (-0.0044 - 0.0138)
0.0048 (-0.0045 - 0.0141)
0.9200 (0.8867 - 0.9534)
0.0062 (-0.0052 - 0.0176)
0.0045 (-0.0041 - 0.0130)
0.0047 (-0.0044 - 0.0139)
0.0045 (-0.0043 - 0.0132)
SSEB
0.0061 (-0.0055 - 0.0178)
0.0099 (-0.0072 - 0.0271)
0.0056 (-0.0051 - 0.0164)
0.0099 (-0.0076 - 0.0274)
0.0133 (-0.0065 - 0.0330)
0.8511 (0.7992 - 0.9029)
0.0078 (-0.0068 - 0.0224)
0.0161 (-0.0096 - 0.0418)
0.0053 (-0.0050 - 0.0156)
IBEL
0.0050 (-0.0046 - 0.0145)
0.0054 (-0.0050 - 0.0158)
0.0053 (-0.0047 - 0.0153)
0.0063 (-0.0054 - 0.0181)
0.0116 (-0.0049 - 0.0281)
0.0057 (-0.0052 - 0.0166)
0.9063 (0.8691 - 0.9435)
0.0061 (-0.0055 - 0.0176)
0.0050 (-0.0047 - 0.0147)
GUAP
0.0060 (-0.0056 - 0.0177)
0.0061 (-0.0058 - 0.0181)
0.0059 (-0.0054 - 0.0173)
0.0063 (-0.0059 - 0.0184)
0.0096 (-0.0065 - 0.0257)
0.0077 (-0.0070 - 0.0225)
0.0070 (-0.0065 - 0.0206)
0.7495 (0.7146 - 0.7845)
0.0058 (-0.0055 - 0.0171)
ICOM
0.0084 (-0.0077 - 0.0244)
0.0083 (-0.0075 - 0.0241)
0.0084 (-0.0077 - 0.0245)
0.0084 (-0.0076 - 0.0244)
0.0091 (-0.0080 - 0.0263)
0.0087 (-0.0079 - 0.0254)
0.0084 (-0.0077 - 0.0244)
0.1271 (0.0819 - 0.1722)
0.6756 (0.6585 - 0.6927)
APIA
0.0052 (-0.0049 - 0.0152)
0.0053 (-0.0047 - 0.0153)
0.0052 (-0.0048 - 0.0151)
0.0057 (-0.0053 - 0.0167)
0.0055 (-0.0052 - 0.0162)
0.0063 (-0.0057 - 0.0183)
0.0068 (-0.0064 - 0.0201)
0.0866 (0.0409 - 0.1323)
0.0052 (-0.0046 - 0.015)
GUAR
0.0073 (-0.0063 - 0.0210)
0.0064 (-0.0058 - 0.0186)
0.0062 (-0.0057 - 0.0180)
0.0065 (-0.0061 - 0.0192)
0.0064 (-0.0062 - 0.0190)
0.0215 (-0.0024 - 0.0455)
0.0071 (-0.0065 - 0.0207)
0.0123 (-0.0086 - 0.0332)
0.0062 (-0.0056 - 0.0181)
BLUM
0.0053 (-0.0049 - 0.0155)
0.0053 (-0.0048 - 0.0155)
0.0052 (-0.0049 - 0.0154)
0.0054 (-0.0050 - 0.0158)
0.0098 (-0.0061 - 0.0257)
0.0062 (-0.0055 - 0.0179)
0.0056 (-0.0052 - 0.0164)
0.0089 (-0.0096 - 0.0275)
0.0052 (-0.0050 - 0.0155)
SJOS
0.0079 (-0.0072 - 0.0231)
0.0079 (-0.0073 - 0.0232)
0.0079 (-0.0073 - 0.0230)
0.0079 (-0.0071 - 0.0230)
0.0080 (-0.0073 - 0.0233)
0.0080 (-0.0073 - 0.0232)
0.0148 (-0.0067 - 0.0364)
0.0084 (-0.0079 - 0.0246)
0.0079 (-0.0073 - 0.0230)
PRUD
0.0059 (-0.0057 - 0.0175)
0.0058 (-0.0053 - 0.0168)
0.0072 (-0.0064 - 0.0208)
0.0062 (-0.0057 - 0.0180)
0.0058 (-0.0054 - 0.0170)
0.0059 (-0.0054 - 0.0172)
0.0057 (-0.0054 - 0.0168)
0.0062 (-0.0057 - 0.0180)
0.0057 (-0.0052 - 0.0165)
PUNI
0.0063 (-0.0058 - 0.0185)
0.0063 (-0.0059 - 0.0185)
0.0062 (-0.0059 - 0.0183)
0.0064 (-0.0058 - 0.0186)
0.0064 (-0.0057 - 0.0185)
0.0062 (-0.0058 - 0.0183)
0.0063 (-0.0059 - 0.0186)
0.0063 (-0.0059 - 0.0185)
0.0062 (-0.0058 - 0.0183)
FOZI
0.0053 (-0.0050 - 0.0156)
0.0054 (-0.0049 - 0.0157)
0.0054 (-0.0049 - 0.0156)
0.0053 (-0.0049 - 0.0156)
0.0053 (-0.0050 - 0.0157)
0.0054 (-0.0052 - 0.0159)
0.0055 (-0.0049 - 0.0159)
0.0053 (-0.0050 - 0.0156)
0.0056 (-0.0052 - 0.0164)
TSAM
0.0051 (-0.0048 - 0.0151)
0.0051 (-0.0048 - 0.0151)
0.0064 (-0.0063 - 0.0191)
0.0053 (-0.0047 - 0.0153)
0.0056 (-0.0051 - 0.0162)
0.0052 (-0.0048 - 0.0153)
0.0052 (-0.0048 - 0.0152)
0.0051 (-0.0049 - 0.0151)
0.0052 (-0.0048 - 0.0151)
Table S5 cont.
FROM
TO
APIA
GUAR
BLUM
SJOS
PRUD
PUNI
FOZI
TSAM
TERE
0.0072 (-0.0056 - 0.0201)
0.0058 (-0.0051 - 0.0167)
0.0055 (-0.0051 - 0.0161)
0.0055 (-0.0049 - 0.0159)
0.0093 (-0.0069 - 0.0255)
0.0054 (-0.0050 - 0.0157)
0.0054 (-0.0048 - 0.0155)
0.0054 (-0.0050 - 0.0157)
RESE
0.0079 (-0.0068 - 0.0227)
0.0075 (-0.0064 - 0.0214)
0.0067 (-0.0061 - 0.0194)
0.0054 (-0.0049 - 0.0156)
0.0089 (-0.0070 - 0.0247)
0.0056 (-0.0050 - 0.0162)
0.0054 (-0.0049 - 0.0157)
0.0054 (-0.0050 - 0.0159)
PASQ
0.0055 (-0.0050 - 0.0160)
0.0077 (-0.0046 - 0.0201)
0.0066 (-0.0047 - 0.0180)
0.0052 (-0.0048 - 0.0152)
0.0196 (-0.0143 - 0.0534)
0.0052 (-0.0047 - 0.0151)
0.0052 (-0.0049 - 0.0153)
0.0051 (-0.0045 - 0.0148)
ANGR
0.0081 (-0.0065 - 0.0228)
0.0061 (-0.0052 - 0.0173)
0.0061 (-0.0053 - 0.0176)
0.0044 (-0.0041 - 0.0130)
0.0055 (-0.0051 - 0.0160)
0.0046 (-0.0044 - 0.0135)
0.0044 (-0.0043 - 0.0131)
0.0046 (-0.0043 - 0.0134)
IGRA
0.0048 (-0.0044 - 0.0140)
0.0050 (-0.0046 - 0.0146)
0.0049 (-0.0046 - 0.0144)
0.0045 (-0.0042 - 0.0133)
0.0057 (-0.0052 - 0.0166)
0.0045 (-0.0042 - 0.0133)
0.0045 (-0.0042 - 0.0133)
0.0046 (-0.0043 - 0.0134)
SSEB
0.0141 (-0.0089 - 0.0370)
0.0184 (-0.0053 - 0.0420)
0.0153 (-0.0046 - 0.0351)
0.0054 (-0.0048 - 0.0156)
0.0059 (-0.0053 - 0.0171)
0.0054 (-0.0050 - 0.0157)
0.0053 (-0.0048 - 0.0155)
0.0053 (-0.0049 - 0.0155)
IBEL
0.0061 (-0.0053 - 0.0175)
0.0060 (-0.0053 - 0.0173)
0.0059 (-0.0053 - 0.0171)
0.0051 (-0.0047 - 0.0150)
0.0051 (-0.0046 - 0.0149)
0.0052 (-0.0047 - 0.0150)
0.0050 (-0.0046 - 0.0146)
0.0050 (-0.0047 - 0.0146)
GUAP
0.0953 (0.0619 - 0.1286)
0.0167 (-0.0116 - 0.0451)
0.0524 (0.0290 - 0.0758)
0.0060 (-0.0054 - 0.0174)
0.0076 (-0.0057 - 0.0209)
0.0059 (-0.0055 - 0.0173)
0.0059 (-0.0056 - 0.0175)
0.0060 (-0.0056 - 0.0176)
ICOM
0.0759 (0.0430 - 0.1087)
0.0094 (-0.0084 - 0.0271)
0.0103 (-0.0082 - 0.0287)
0.0084 (-0.0075 - 0.0243)
0.0084 (-0.0075 - 0.0244)
0.0084 (-0.0077 - 0.0246)
0.0085 (-0.0077 - 0.0246)
0.0084 (-0.0076 - 0.0244)
APIA
0.7125 (0.6774 - 0.7477)
0.0524 (0.0097 - 0.0952)
0.0774 (0.0495 - 0.1053)
0.0051 (-0.0047 - 0.0150)
0.0052 (-0.0048 - 0.0152)
0.0052 (-0.0048 - 0.0152)
0.0052 (-0.0048 - 0.0152)
0.0052 (-0.0049 - 0.0153)
GUAR
0.0172 (-0.0106 - 0.0449)
0.7777 (0.7388 - 0.8165)
0.0943 (0.0694 - 0.1192)
0.0061 (-0.0057 - 0.0180)
0.0063 (-0.0059 - 0.0185)
0.0061 (-0.0058 - 0.0180)
0.0062 (-0.0057 - 0.0181)
0.0062 (-0.0058 - 0.0181)
BLUM
0.0082 (-0.0076 - 0.0240)
0.1476 (0.1170 - 0.1782)
0.7606 (0.7346 - 0.7865)
0.0053 (-0.0048 - 0.0153)
0.0056 (-0.0051 - 0.0163)
0.0052 (-0.0048 - 0.0153)
0.0052 (-0.0049 - 0.0154)
0.0052 (-0.0049 - 0.0153)
SJOS
0.0083 (-0.0076 - 0.0241)
0.1217 (0.0844 - 0.1590)
0.0848 (0.0482 - 0.1214)
0.6750 (0.6592 - 0.6908)
0.0078 (-0.0069 - 0.0225)
0.0079 (-0.0072 - 0.0229)
0.0079 (-0.0071 - 0.0229)
0.0079 (-0.0072 - 0.0229)
PRUD
0.0067 (-0.0056 - 0.0190)
0.0076 (-0.0055 - 0.0207)
0.0073 (-0.0052 - 0.0198)
0.0057 (-0.0052 - 0.0166)
0.9015 (0.8620 - 0.9411)
0.0056 (-0.0055 - 0.0168)
0.0056 (-0.0054 - 0.0167)
0.0055 (-0.0051 - 0.0162)
PUNI
0.0064 (-0.0058 - 0.0186)
0.0064 (-0.0057 - 0.0184)
0.0063 (-0.0060 - 0.0186)
0.0063 (-0.0058 - 0.0184)
0.0064 (-0.0059 - 0.0186)
0.7632 (0.7387 - 0.7878)
0.1421 (0.1114 - 0.1728)
0.0063 (-0.0059 - 0.0185)
FOZI
0.0054 (-0.0050 - 0.0158)
0.0054 (-0.0051 - 0.0160)
0.0054 (-0.0051 - 0.0160)
0.0054 (-0.0052 - 0.0159)
0.0054 (-0.0048 - 0.0157)
0.1034 (0.0828 - 0.1240)
0.8156 (0.7893 - 0.8419)
0.0054 (-0.0051 - 0.0159)
TSAM
0.0052 (-0.0047 - 0.0152)
0.0052 (-0.0049 - 0.0152)
0.0051 (-0.0048 - 0.0151)
0.0051 (-0.0049 - 0.0151)
0.2486 (0.2122 - 0.2849)
0.0052 (-0.0048 - 0.0152)
0.0052 (-0.0048 - 0.0151)
0.6721 (0.6615 - 0.6826)
Table S6. Population demography results in 17 populations of Tetragonisca angustula. Bottlenecks based
on microsatellite data were estimated by Wilcoxon sign test P-value and by the distribution of the allelic
frequencies (DAF). Population abbreviations as in Table 1.
TERE
RESE
PASQ
ANGR
IGRA
SSEB
IBEL
GUAP
ICOM
APIA
GUAR
BLUM
SJOS
PRUD
PUNI
FOZI
TSAM
Wilcoxon P-value
0.8799
0.1392
0.6523
0.5508
0.2886
0.9126
0.9263
0.8174
0.4829
0.8799
0.9492
0.9126
0.8398
0.9663
0.9983
0.9983
0.9998
DAF
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution
normal L-shaped distribution