ANTI-LEPTOSPIROSIS AGGLUTININ RESEARCH IN THE SERUM OF RIG PULLING EQUINE IN Porto Alegre/RS – Brasil Camila Serina Lasta, Luciana Paula Merini1, Simone Tostes de Oliveira, Vanessa Galimberti, Viviane Pedralli, Alexandra Tiso Cormelato, José Antonio Simões Pires Neto, Carlos Afonso de Castro Beck, Félix H. D. González, Luiza Aita de Lemos Introduction: Leptospirosis is one of the main world zoonosis and Brazil is considered an endemic area for the disease, which presents serious risk to public health. Equine leptospirosis is a disease caused by different serum varieties of Leptospira interrogans, and however it may cause sereve illness, abortion and other reproductive tract problems, most infections have an asymptomatic characteristic. Equine can excrete viable leptospira in urine for approximately 14 weeks, which make them an important resorvoir for the transmission of the disease to other animal and to men. Objectives: To investigate the ocurrence of antibodies anti Leptospira interrogans in equine used for rig pulling when collecting recyclable trash in Porto Alegre – RS. Methods: 125 serum samples were tested for the presence of agglutinins agains 13 serum varieties of Leptospira interrogans through the plate micro agglutination test, being considered as a positive result, agglutinations equal or above 1: 100. Results: 92 samples (73.6 %) were positive, being: 50 samples (54.34 %) positive to the serum variety Pomona, 48 samples (52.17 %) to Icterohaemorrhagie, 16 samples (7.39 %) to Australis, 15 samples (16.3 %) to Copenhageni, 9 samples (9.78 %) to Autumnalis, 6 samples (6.52 %) to Hardjo, 6 samples (6.52 %) to Wolffi, 5 samples (5.43 %) to Heb, 2 samples (2.17 %) to Grippothyphosa, 2 samples (2.17 %) to Pyrogens, 1 sample (1.08 %) to Canicola and 1 sample (1.08 %) to Tarassovi, considering that 73 samples presented antibodies against two or mora serum varieties. No agglutination was observed to the serum variety Bratislava. Discussion and Conclusions: In Porto Alegre, equine leptospirosis can be considered endemic. Considering that none of the animals analised presente clinical signs suggesting Leptospirosis, the equine used to pull rigs to collect recyclable trash play a very import part as reservoirs to the disease, since they cover great distances daily and can carry the microorganism from one place to another within the city. 1 Universidade Federal do Rio Grande do Sul (UFRGS). Author adress: 9090, Av. Bento Gonçalves, Agronomia, ZIP Code: 91540-000, Porto Alegre – RS/Brazil (BRA). E-mail: [email protected]