Short communication
Phylogeography of Drosophila subobscura from North Atlantic Islands
inferred from mtDNA A+T rich region sequences
António Brehm1*, D. James Harris2, Mariano Hernández3, José A. Perez3, José M.
Larruga3, Francisco M. Pinto3, and Ana M. González3
1 Centre of Macaronesian Studies, University of Madeira, Campus of Penteada,
9000-390 Funchal, Portugal.
2 CIBIO, ICETA-U.P., Campus Agrario de Vairão, 4485-661 Vila do Conde, Portugal
3 Department of Genetics, University of La Laguna, Tenerife 38271, Spain
* Corresponding author: Email [email protected]
Running title: Phylogeography of D. subobscura populations from Atlantic Islands
Keywords: Drosophila subobscura, A+T rich region, phylogeography, Madeira, Azores,
Canary Island
1
Complementary Material
Table 1. A+T rich region partial sequences (P) found in 103 individuals of D. subobscura belonging to 14 localities. The codes for the regions
are as in Figure 1 of the main article. Nucleotide numbers refer to sub-regions R7-R8 of the complete A+T rich region sequence of D.
subobscura that has been described elsewhere (AC AJ132899-132900, Brehm et al. 2001). A dot indicates the same base as in partial
sequence 1. Indels are indicated by a dash. Nucleotide position 586 refers to a high variable stretch already identified in R7 region (Brehm et al.
2001).
555555555555555555555555666666666667777788888999999
012377778888888888888899011344445660078915677000224
737404890466666666668957224312562382319129608358450
P1
P2
P3
P4
P5
P6
P7
P8
P9
P10
P11
P12
P13
P14
P15
TTAATATAAA----------TAATAATATTTT-TATAATAAAATTTTTATT
................TATA....-....C....T..........CC..C.
...........................G................A......
..........................C........................
..................................T..........C...CC
...........................................A.......
.....................T.............................
.A...................TC......C....T..G...G...C.C...
.A...................TC......C....T..G...G...C....C
.A....................C......C....T..G...G...C....C
.....................T............T..G...G...C....C
........................-....C....T..........CC....
.A...................TT......C....T..G...G...C....C
.A....................C......C....T..G.......C....C
........................................G..........
A
Z
1
A
Z
2
3
1
1
Canary isl.
A
Z
3
4
1
M
A
C
A
1
C
A
2
4
C
A
3
1
C
A
4
1
Iberia
C
A
5
1
A
G
I
B
1
2
I
B
2
11 4
1
2
1
1
1
3
4
1
1
1
I
B
3
2
1
2
1
1
1
3
1
1
I
B
4
3
Total
Azores
Isl.
34
1
1
3
1
1
3
1
6
5
1
1
1
5
2
1
P16
P17
P18
P19
P20
P21
P22
P23
P24
P25
P26
P27
P28
P29
P30
P31
P32
P33
P34
P35
P36
P37
P38
P39
P40
P41
P42
P43
.............................C....T..G...G...C....C
.A....................C......C....T.TG...G...C....C
....A.........TATATG....-....C....T..........CC..C.
....A.........TATATG....-....C....T................
.....T..................-....C....T................
A.....---.......TATA....-....C....T..........C....C
.....................................G.............
................TATA....-....C....T..........CC...C
..........TATATATATA....-...CC....T..........CC..C.
........................-....C....T..........CC..C.
..............TATATG....-....C....T................
..............................A.A..................
..............TATATG....-....C....T..........CC..C.
..................................T................
.......................A...........................
.........G.........................................
.........................C.....A...................
..................................................C
.................................C................C
..G....................................G...........
................................A..................
................................................G..
.............................C....T..........C.....
.......TT. TATATATATA....-....C....T..........C.....
...T...TT. TATATATATA....-....C....T..........C.....
........T. TATATATATA....-....C....T..........C.....
........T. TATATATATA....-....C....T..........CC..CC
....................C..............................
1
2
1
1
1
1
1
1
1
2
1
1
2
1
1
1
1
1
2
1
1
1
1
2
1
1
1
1
1
1
1
1
1
2
1
1
1
1
2
1
1
2
2
1
1
1
2
1
1
3
1
1
2
1
1
1
1
2
1
1
2