Short communication Phylogeography of Drosophila subobscura from North Atlantic Islands inferred from mtDNA A+T rich region sequences António Brehm1*, D. James Harris2, Mariano Hernández3, José A. Perez3, José M. Larruga3, Francisco M. Pinto3, and Ana M. González3 1 Centre of Macaronesian Studies, University of Madeira, Campus of Penteada, 9000-390 Funchal, Portugal. 2 CIBIO, ICETA-U.P., Campus Agrario de Vairão, 4485-661 Vila do Conde, Portugal 3 Department of Genetics, University of La Laguna, Tenerife 38271, Spain * Corresponding author: Email [email protected] Running title: Phylogeography of D. subobscura populations from Atlantic Islands Keywords: Drosophila subobscura, A+T rich region, phylogeography, Madeira, Azores, Canary Island 1 Complementary Material Table 1. A+T rich region partial sequences (P) found in 103 individuals of D. subobscura belonging to 14 localities. The codes for the regions are as in Figure 1 of the main article. Nucleotide numbers refer to sub-regions R7-R8 of the complete A+T rich region sequence of D. subobscura that has been described elsewhere (AC AJ132899-132900, Brehm et al. 2001). A dot indicates the same base as in partial sequence 1. Indels are indicated by a dash. Nucleotide position 586 refers to a high variable stretch already identified in R7 region (Brehm et al. 2001). 555555555555555555555555666666666667777788888999999 012377778888888888888899011344445660078915677000224 737404890466666666668957224312562382319129608358450 P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12 P13 P14 P15 TTAATATAAA----------TAATAATATTTT-TATAATAAAATTTTTATT ................TATA....-....C....T..........CC..C. ...........................G................A...... ..........................C........................ ..................................T..........C...CC ...........................................A....... .....................T............................. .A...................TC......C....T..G...G...C.C... .A...................TC......C....T..G...G...C....C .A....................C......C....T..G...G...C....C .....................T............T..G...G...C....C ........................-....C....T..........CC.... .A...................TT......C....T..G...G...C....C .A....................C......C....T..G.......C....C ........................................G.......... A Z 1 A Z 2 3 1 1 Canary isl. A Z 3 4 1 M A C A 1 C A 2 4 C A 3 1 C A 4 1 Iberia C A 5 1 A G I B 1 2 I B 2 11 4 1 2 1 1 1 3 4 1 1 1 I B 3 2 1 2 1 1 1 3 1 1 I B 4 3 Total Azores Isl. 34 1 1 3 1 1 3 1 6 5 1 1 1 5 2 1 P16 P17 P18 P19 P20 P21 P22 P23 P24 P25 P26 P27 P28 P29 P30 P31 P32 P33 P34 P35 P36 P37 P38 P39 P40 P41 P42 P43 .............................C....T..G...G...C....C .A....................C......C....T.TG...G...C....C ....A.........TATATG....-....C....T..........CC..C. ....A.........TATATG....-....C....T................ .....T..................-....C....T................ A.....---.......TATA....-....C....T..........C....C .....................................G............. ................TATA....-....C....T..........CC...C ..........TATATATATA....-...CC....T..........CC..C. ........................-....C....T..........CC..C. ..............TATATG....-....C....T................ ..............................A.A.................. ..............TATATG....-....C....T..........CC..C. ..................................T................ .......................A........................... .........G......................................... .........................C.....A................... ..................................................C .................................C................C ..G....................................G........... ................................A.................. ................................................G.. .............................C....T..........C..... .......TT. TATATATATA....-....C....T..........C..... ...T...TT. TATATATATA....-....C....T..........C..... ........T. TATATATATA....-....C....T..........C..... ........T. TATATATATA....-....C....T..........CC..CC ....................C.............................. 1 2 1 1 1 1 1 1 1 2 1 1 2 1 1 1 1 1 2 1 1 1 1 2 1 1 1 1 1 1 1 1 1 2 1 1 1 1 2 1 1 2 2 1 1 1 2 1 1 3 1 1 2 1 1 1 1 2 1 1 2