1 Curriculum vitae Full Name: José Alexandre da Costa Perdigão e Cameira Leitão Nationality: Portuguese Work address: Instituto de Investigação Científica Tropical CVZ/IICT ‐ Faculdade de Medicina Veterinária, Avenida da Universidade Técnica 1300‐477 Lisboa PORTUGAL Phone: 213652800 (ext. 1361) Email: [email protected] Fax: 213652869 Academic degrees: 2008 ‐ Agregação, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa 1999 ‐ PhD equivalence, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa 1998 ‐ PhD, Doctoraat in de Wetenschappen, Vrije Universiteit Brussel (VUB), Belgium 1991 ‐ MSc, Medicina Veterinária e Zootecnia Tropicais, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa 1985 ‐ Degree in Veterinary Medicine, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa Positions held: March 2008 ‐ present date: Investigador Auxiliar c/ Agregação Instituto de Investigação Científica Tropical, Lisboa. November 1996 ‐ March 2008: Investigador Auxiliar, Instituto de Investigação Científica Tropical, Lisboa. September 1991 ‐ November 1996: Assistente de Investigação, Instituto de Investigação Científica Tropical, Lisboa. September 1988 ‐ September 1991: Assistente Estagiário de Investigação, Instituto de Investigação Científica Tropical, Lisboa. 2 May 1987 ‐ August 1988: Fellowship, Instituto Gulbenkian de Ciência, Oeiras. May 1986 ‐ March 1987: Research Trainee, Laboratório Nacional de Investigação Veterinária, Lisboa. Domain of specialization: Veterinary infection and immunology Current research interests: African swine fever, bovine besnoitiosis, equine piroplasmosis Peer reviewed publications in international scientific journals (last 5 years): ‐ Portugal, R., Leitão, A., Martins, C. (2009). Characterization of African swine fever virus IAP homologue expression in porcine macrophages infected with different virulence isolates. Veterinary Microbiology [Epub ahead of print] DOI:10.1016/j.vetmic.2009.04.030 ‐ Portugal, R., Leitão, A., Martins, C. (2009). Apoptosis in porcine macrophages infected in vitro with African swine fever virus (ASFV) strains with different virulence. Archives of Virology, 154: 1441‐1450. DOI: 10.1007/s00705‐009‐0466‐x ‐ Gallardo, C., Reis, A.L., Kalema‐Zikusoka, G., Malta, J., Soler, A., Blanco, E., Parkhouse, R.M., Leitão, A. (2009). Recombinant antigen targets for serodiagnosis of African swine fever. Clinical and Vaccine Immunology, 16:1012‐1020. DOI: 10.1128/CVI.00408‐08 ‐ Gil, S., Sepúlveda, N., Albina, E., Leitão, A., Martins, C. (2008). The low‐virulent African swine fever virus (ASFV/NH/P68) induces enhanced expression and production of relevant regulatory cytokines (IFNalpha, TNFalpha and IL12p40) on porcine macrophages in comparison to the highly virulent ASFV/L60. Archives of Virology, 153:1845‐1854. DOI: 10.1007/s00705‐008‐
0196‐5 ‐ Reis, A.L., Parkhouse, R.M.E, Penedos, R., Martins, C., Leitão, A. (2007). A systematic analysis of longitudinal serological responses of pigs experimentally infected with African swine fever Virus. Journal of General Virology, 88: 2426‐2434. DOI: 10.1099/vir.0.82857‐0 ‐ Cortes H.C., Mueller, N., Esposito, M., Leitao, A., Naguleswaran, A., Hemphill, A. (2007). In vitro efficacy of nitro‐ and bromo‐thiazolyl‐salicylamide compounds (thiazolides) against Besnoitia besnoiti infection in Vero cells. Parasitology, 134:975‐985. DOI: 10.1017/S0031182007002417 ‐ Cortes, H.C., Reis, Y., Gottstein, B., Hemphill, A., Leitao, A., Muller, N. (2007). Application of conventional and real‐time fluorescent ITS1 rDNA PCR for detection of Besnoitia besnoiti infections in bovine skin biopsies. Veterinary Parasitology, 146(3‐4): 352‐356. DOI: 10.1016/j.vetpar.2007.03.003 3 ‐ Reis Y, Cortes H, Viseu Melo L, Fazendeiro I, Leitao A, Soares H. (2006). Microtubule cytoskeleton behavior in the initial steps of host cell invasion by Besnoitia besnoiti. FEBS Letters. 580(19):4673‐4682. DOI:10.1016/j.febslet.2006.07.050 ‐ Cortes H.C., Nunes S, Reis Y, Staubli D, Vidal R, Sager H, Leitao A, Gottstein B. (2006). Immunodiagnosis of Besnoitia besnoiti infection by ELISA and Western blot. Veterinary Parasitology. Veterinary Parasitology 141(3‐4): 216‐225. DOI: 10.1016/j.vetpar.2006.05.023 ‐ Cortes H.C., Reis Y, Waap H, Vidal R, Soares H, Marques I, Pereira da Fonseca I, Fazendeiro I, Ferreira M.L., Caeiro V, Shkap V, Hemphill A, Leitao A. (2006). Isolation of Besnoitia besnoiti from infected cattle in Portugal. Veterinary Parasitology. 141(3‐4):226‐233. DOI: 10.1016/j.vetpar.2006.05.022 ‐ Basto, A.P., Nix, R.J., Boinas, F., Mendes S., Silva, M.J., Cartaxeiro, C., Portugal, R.S., Leitão, A., Dixon, L.K., Martins, C. (2006). The kinetics of African swine fever virus (ASFV) infection in Ornithodoros erraticus ticks. Journal of General Virology, 87:1863‐1871. DOI: 10.1099/vir.0.81765‐0 ‐ Basto, A., Portugal R.S., Cartaxeiro C., Boinas F., Dixon L., Leitão A. and Martins C. (2006). Development of a Nested PCR and its Internal Control for the detection of African swine fever virus (ASFV) in Ornithodoros erraticus. Archives of Virology, 151: 819‐826. DOI: 10.1007/s00705‐005‐0654‐2 ‐ Cortes, H., Leitão, A., Vidal, R., Vila‐Viçosa, M. J., Ferreira, M. L., Caeiro, V., and Hjerpe, C. A. (2005). Bovine Besnoitiosis in Portugal. The Veterinary Record, 157: 262‐264.