Evaluation of antibacterial activity of latex of Sapium
glandulatum native Cerrado
Possamai, M. M.[1], Lima, M. F. [2], Souto, P. C. S. [3]
1,2,3
Universidade Federal de Mato Grosso, Campus Universitário do Araguaia, Brazil.
Studies have shown that some plant species of Cerrado medicinal properties [1]
[2]
, antibacterial [1], antitumor [3], among others [4]. However, most of them have not been
chemically or biologically evaluated [2]. The aim of this study was to assess the
feasibility of antibacterial activity using Staphylococcus aureus in vitro in the presence
of latex Sapium glandulatum, as part of a previous evaluation, aimed at implementing
this as a biomaterial. For the test, the latex was extracted and diluted in PBS. Use petri
dishes with 60 mL of medium Miller-Hinton agar, by the spread plate method, and on
each plate four wells drilled and placed 10 µL of latex, then incubated for a period of 24
hours under aerobic conditions. The reading was done with a caliper and measuring the
size of inhibition zone [5], and gram stain observation with optical microscope. It was
found that the latex antibacterial activity and therefore a possible candidate for the
development of a new biomaterial for further application in the treatment of diseases
caused by these organisms.
Key-words: latex, biomaterial, antibacterial, Sapium glandulatum, Staphylococcus
aureus
References:
[1]
COSTA, E.S. et al. Antimicrobial activity of some medicinal plants of the Cerrado,
Brazil. Phytother. Res. 22(5):705-707. 2008.
[2]
MELO E SILVA, F. et al. Evaluation of the antifungal potential of Brazilian Cerrado
medicinal plants. Mycoses. 52:511–517. 2009.
[3]
MESQUITA, M.L. et al. Cytotoxic activity of Brazilian Cerrado plants used in
traditional medicine against cancer cell lines. Journal of Ethnopharmacology.
123:439–445. 2009.
[4]
RODRIGUES, E. Plants of restricted use indicated by three cultures in Brazil
(Caboclo-river dweller, Indian and Quilombola). Journal of Ethnopharmacology.
111:295–302. 2007.
[5]
NCCLS. Performace Standarts for Antomicrobial disk susceptilly tests: approved
standart. 8 ed. NCCLS Document M2 – A8. Pesylvania, USA, 2003.
[email protected] UFMT – Universidade Federal de Mato Grosso, CUA
– Campus Universitário do Araguaia. End.: MT – 100; KM: 3,5. Pontal do Araguaia,
MT. Brasil.