Biomaterials
Biodegradables
Biomimetics
For more Information go to www.3bs.uminho.pt
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
3B´s is a member of the PT Associated Laboratory
IBB – Institute for Biotechnology and Bioengineering
For more Information go to www.ibb.pt
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
3B´s RESEARCH GROUP (89)
Other PhD Holders (21)
Staff (4)
Alexandra Marques
- Rui L. Reis (Director)
Ana Grenha
- João F. Mano
Ana Frias
- Nuno M. Neves
António Salgado
- Natália Alves
Carlos Viegas
Erkan Baran
Gabriela Silva
Technical Staff (8)
Helena Azevedo
Ariana Santos
Isabel Dias
Bárbara Barroso
Iva Pashkuleva
Berta Almeida
Johan Benesch
Filipe Ribeiro
Luciano Boesel
Liliana Gomes
Maria Manuela Gomes
Ricardo Gomes
Mazen Tamer
Tânia Delalande Alves
Ricardo Pires
Virgínia Araújo
Rita Serra
Rui Amandi de Sousa
Sandra Sampaio
Susana Martins
Tiago Silva
Zhongkui Hong
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
PhD Students/ Other Full time Researchers (56)
Adriano Pedro
Albino Martins
Ana Mendes
Ana Pinheiro
Ana Oliveira
Ana Sousa
Ana Pinto
Anabela Pinto
Barbara Bertini
Brecht Claes
Bruno Ferreira
Carolina Fernandez
Catarina Alves
Cornelia Brunner
Daniela Coutinho
Elisabete Pinho
Elizabeth Balmayor
Emanuel Fernandes
Esther Garcia
Helena Lima
Isabel Leonor
Ivo Aroso
Jessica Grech
Joana Magalhães
João Oliveira
Joaquim Ferreira
Joaquim Oliveira
José Rodriguez
José Pereira
José Júnior
Kadriye Tuzlakoglu
Leandro Gardel
Márcia Rodrigues
Marina Santos
Marta Silva
Patrícia Baptista
Paula Sol
Paula Pérez
Paulo Bessa
Ricardo Silva
Rogério Sousa
Rosa Bessada
Sandra Osório
Sangwong Chung
Satyabrata Gosh
Sílvia Pereira
Silvienne Novikoff
Simone Silva
Susana Santos
Teresa Silva
Tírcia Santos
Tommaso Rada
Vitor Espírito Santo
Vitor Gutierrez
Vítor Silva
Wojciech Szymczyk
BACKGROUND OF 3B´s RESEARCHERS
Materials & Polymer Engineering
10%
3%
20%
Chemistry & Chemical Engineering
Biology & Biochemistry
9%
Biotechnology and Biomedical Engineering
Medicine
Management
25%
33%
GENDER: Female 49% and Male 51%
Total: 89 (Fev 2007)
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
EU FUNDED PROJECTS
Marie Curie EST - ALEA JACTA EST
STREP – HIPPOCRATES
NoE – EXPERTISSUES
Marie Curie SCF - InVENTS
IP – GENOSTEM
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
•
•
•
•
STREP – WaCheUp
STREP - NANOPHOTOTECH
Marie Curie EST JoinTEd
Marie Curie: POLYSTEM
3B´s RESEARCH GROUP FUNDING
(ONGOING PROJECTS)
FCT Fellowships
(PhD and PostDoc)
18%
Projects Directly
Funded by
Industry
6%
3B´s Present Budget
of around 10 M€
Coordinator (PI) of
around 30 M€ in
Projects
Projects Funded
by European R&D
Programmes
57%
Projects Funded
by National FCT
Programme
19%
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
SOME BOOKS AND SPECIAL ISSUES EDITED by 3B’s
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
NEW JOURNAL - TERM
Editor-in-Chief
Professor Rui L. Reis
Associate Editors
Professor Samuel I. Stupp
Dr Hajime Ohgushi
Professor Anthony Atala
A new leading journal from
John Wiley and Sons Ltd
Launching Feb 2007
www.interscience.wiley.com/journal/term
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
European Institute of Excellence on Tissue
Engineering and Regenerative Medicine Research
Headquarters and the Home for 3B´s Research – Late 2007
AvePark, Taipas, Minho, Portugal
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
3B´s SCIENTIFIC PROGRAM
• T1 - Development and modification of natural origin
materials, including exploitation of marine origin resources
• T2 - Polymer science applied to the development of new
materials
• T3 - Characterization of biomaterials under dynamic loads
• T4 - Behaviour in simulated physiological solutions and
degradation mechanisms
• T5 - Processing and characterization of biodegradable
systems including the development of bioactive, bioinert
composites and nanostructured composites
• T6 – Nanobiotechnolgy for Health and Nanomedicine
• T7- Surface modification of biomaterials and micro/nano/bio
interactions
• T8 - Bioactivity, biomineralization, biomimetic coatings and
bioceramics
• T9- Biomaterials-protein interactions
• T10 - Production of porous biomaterials
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
3B´s SCIENTIFIC PROGRAM
• T11 - Systems for the controlled release of bioactive agents,
including dendrimers and nano-particle release systems
• T12 - Hydrogels and degradable bone cements
• T13 - Membranes and wound dressings of natural origin
• T14- Tissue engineering of bone, cartilage, skin and
neurological tissues
• T15 – Stem Cells: Sources, Isolation, Expansion and
Differentiation
• T16 – Dynamic culturing and bioreactors in Tissue
Engineering
• T17 - Biocompatibility and immunological responses to
biomaterials
• T18 – In-vivo animal tests
• T19 – New products/applications of added value for cork and
cork based products (long-term partnership with the World
leader – Corticeira AMORIM SGPS)
• T20 – Projects Management & Quality Assurance
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
THE CREATION OF A NEW TISSUE/ORGAN
In vitro
Expansion
Biopsy
Seeding
on
Scaffold
Implantation
Extended Culture
in Bioreactor
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
CHITIN / CHITOSAN
Chitin is insoluble in most common solvents, but can be converted to
chitosan which is soluble in diluted acids and possesses unique
properties that make it suitable to be used as a biomaterial.
CHITIN
CH2OH
CH2OH
H
OH
H
H
O
H
NHCOCH3
GlcNAc
H
OH
H
H
O
Deacetylation
H
NHCOCH3
GlcNAc
CH2OH
CH2OH
O
H
O
H
CHITOSAN
NaOH, temperature
H
OH
H
n
H
H
NH2
O
H
O
H
O
H
OH
H
H
NH2
O
H
+ CH3COONa
n
Chitosan
Scaffolds
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
STEM /PROGENITOR CELLS
• EMBRYONIC
• BONE MARROW
• ADIPOSE TISSUE
• UMBILICAL CORD
• AMNIOTIC MEMBRANE
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
SOME EXAMPLES OF NANOMEDICINE RESEARCH
Biomaterials
Biodegradables
Biomimetics
For more Information go to www.3bs.uminho.pt
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
ELECTROSPINNING & NANO-MESHES
5 um
Pump
High
Syringe Voltage
Power
Supply
Solution
Distan
ce
Collector
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
TUBULAR STRUCTURES & HOLLOW NANOFIBERS
2 um
2 um
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
PREPARATION OF MICRO- and NANO- FIBER COMBINED SCAFFOLDS
Electrospinning: production of fibers in nano-size by electrically
charging a suspended droplet of polymer solution or melt.
Experimental Setup
3D deposition of SPCL nanofibers onto an
SPCL macro fiber-mesh
polymer solution
Taylor cone
High voltage
power supply
charged fibers
collection plate
Micro- and Nano-fiber Combined Scaffold
Combine the advantageous features of nano and macro fibers.
Enhancement of the surface area of tissue engineering scaffolds while
maintaining their porosity.
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
GROWTH AND VIABILITY
HUVEC cells
7 days
Nano/micro fibers combined scaffold
Green – Calcein-AM staining
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
Control
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
50 μm
In - Situ AFM Æ SEVA - C + 30% HA
0h
8h
125 h
120 h
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
126 nm
24 h
128 h
Biomimetic Ca-P Coated microspheres
Untreated
12 h (100 strokes/min) / 100ºC
24 h (100 strokes/min) / 60ºC
10 µm
1 µm
1 µm
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
10 µm
10 µm
10 µm
1 µm
1 µm
10 µm
After soaking in SBF for 7 days
NEW DEVELOPMENT: BIOMIMETIC COATING OF NANO-MESHES
NT-2d
NT-4d
2 µm
CaPT3h
NT-7d
2 µm
CaPT6h
5 µm
CaPT4d
CaPT2d
5 µm
CaPT7d
5 µm
5 µm
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
2 µm
5 µm
DENDRIMERS CONJUGATED WITH NATURAL-BASED POLYMERS
+
PAMAM dendrimers
Carboxymethyl-chitosan
CMC/PAMAM
Dendronized polymer
AIM:
Development
and
characterization
novel
carboxymethyl-chitosan/poly
(amidoamine) (CMC/PAMAM) dendronized polymers (natural-based polymers
+ dendrimers).
These nanocarriers may find applications in the intracellular controlled drug
delivery aimed at modulate the cells behaviour, namely differentiation and
proliferation of stem cells, which is one of the key issues in the
comprehensive research of stem cell-based tissue engineering.
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
INTERNALIZATION OF CMC/PAMAM-FITC NANOPARTICLES
INTERNALIZATION OF CMC/PAMAM-FITC NANOPARTICLES
In SaOs-2 Cell Line - AFTER 14 Days
SaOs-2 with CMC/PAMAM-FITC
after 14 days
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
MICROPATTERNING OF BIODEGRADABLE MEMBRANES
osteoblast cell response to micropatterned PCL membranes
24 h
25μm
15μm
50μm
25μm
48 h
Non-patterned
membrane
96 h
25μm
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
25μm
MAIN INTERNATIONAL COLLABORATIONS
–
CSIC - Madrid, SP (Julio San Roman)
–
Johannes Guttenberg University – Mainz, D (James Kirkpatrick)
–
U. Liverpool, UK, (John Hunt)
–
U. Twente, NL, (Clemens van Blitterswijk)
–
Tissue Engineering Res. Centre - Tsukuba, Japan (Atsuo Ito)
–
National Univ. Singapore, SG, (Dietmar Hutmacher)
–
U. Hacettepe, TK (Ehran Piskin)
–
Rice University, USA (Tony Mikos)
–
Univ. Pennsylvannia & Thomas Jefferson Univ., USA (P. Ducheyne, I. Shapiro)
–
University of Toronto, Canada (John Davies)
–
Kyoto University and Chubu Univ., Japan (Tadashi Kokubo)
–
Univ. Texas at San Antonio, USA (Mauli Agrawal)
–
Ludwig Boltzmann Institute, Trauma – Vienna (Heinz Redl)
–
Red Cross Upper Austria, Linz (Christian Gabriel)
–
Sheffield University, UK, (Paul Hatton)
LABORATÓRIO ASSOCIADO
INSTITUTO DE BIOTECNOLOGIA E BIOENGENHARIA
–
Northwestern University (Sam I. Stupp)
–
AIST, Osaka (Hajime Ohgushi)
–
Harvard University (David Mooney)
–
Georgia Tech. (Andres Garcia)
–
Univ. la Sapienza, Rome (Paolo Bianco)
–
Univ. Hospital Basel (Ivan Martin)
–
Keele University (Alicia El Haj)
–
University Genova (Ranieri Cancedda)
–
University of Trento (Claudio Migliaresi)
–
University of Kyoto (Y. Tabata)