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Comparative effect of a phytotherapeutic product from Brassica ssp as cicatrizing
Silva, M. B.1; Silva, C. A.2; Malaquias, L.C.C.3; Sarandy, M. M.1; Freire, M. C. M.4; Antunes. F. R.2; Costa, A.
V. S.1
1
Universidade Vale do Rio Doce. Faculdade de Ciências Agrárias. Cep 35020-500. Governador Valadares, MG,
Brazil. E-mail: [email protected]; 2Faculdade de Ciências da Saúde, Universidade Vale do Rio Doce, MG,
Brazil. 3Núcleo de Pesquisas em Imunologia, Universidade Vale do Rio Doce, MG, Brazil; 4Faculdade de Ciências,
Educação e Letras, Universidade Vale do Rio Doce, MG, Brazil.
ABSTRACT: Comparative effect of a phytotherapeutic product from Brassica sp as cicatrizing. The
use of the phytotherapy is very old, and now it is expanding all over the world. New phytotherapeutic
products have to have its effects certificated in the scientific bases, including toxicological studies, what
justifies the use of biological experiments. In this work the effect of a phytotherapic product from Brassica
sp (Phytoderminâ) was compared to a standard industrialized product (Kollagenaseâ) on the wounds
cicatrization rates in surgically induced animals. The tests were carried out using nine animals (Cavia
cobaya), weighing around 500 to 600 grams. The hair of the lateral right of each animal was removed
mechanically. All animals received a local anesthesia with 0.5 mL of lidocain 2% plus fenilferin 1:1000.
After that, a surgical incision of approximately 2.0 cm2 of diameter was made. The animals were treated
with the ointments of the two products (Phytoderminâ and Kollagenaseâ), applied twice a day until the end
of the cicatrization process. The control animals group was treated only with physiologic saline. The
experiment was made using three animals per group. The cicatrization rate was accompanied by measuring
the length and the width of the wounds daily. The cicatrization of the wounds takes place in approximately
twenty days. In the control animals treated with none of the tested products it was observed a delay in the
cicatrization process. With the data of cicatrization evolution, it was possible to adjust one curve for each
treatment, with aid of the lineal model. The values of the rates estimated in each treatment were compared
using Tukey test (p=0.05). The cicatrization rates obtained with use of Phytoderminâ and with Kollagenaseâ
didn’t differ from each other, although both rates was larger than the one observed in the control animals,
suggesting that the phytotherapeutic product (Phytoderminâ) used to heal the wounds had similar effect of
the commercial industrialized product (Kollagenaseâ).
Key words: phytotherapy, Cavia cobaya, plant extract, wound healing.
INTRODUCTION
The human kind has been using plants with
therapeutic purposes since remote times, and its use
is expanding now all over the world. However, for
consume of medical plants settle down is necessary
the use of experimentation and scientific validation of
the current therapeutic dose and monitoring the
collateral effects of its use (Elizabetsky, 1987). These
currents need justifying the accomplishment of
biological tests.
According Clark (1993), the events of the
wound cicatrization can be divided in three phases
that are not mutually excluding, but put upon in the
time. These phases are nominated of inflammatory
stage, with a pick in the first hours after the injury,
preceded by granulation and later on for epithelization
(Politis & Dmytrowich, 1998). The cicatrization
process is characterized by the fouling of the wound
and closed by the scar. However, these stages can
be altered by the presence or absence of some
Recebido para publicação em agosto/2004
Aceito para publicação em julho/2006
bacteria, wound type (open or close), degree of
sanguine supply, type of tissue and others one (Manjo
& Joris, 1996).
From the natural substances normally used
for the cutaneous wound repair, it is possible to stand
out the honey (Oryan & Zaker, 1998), the proplis
(Silveira & Raiser, 1995), and Aloe Vera’s leaves
(Chitahra et al., 1995). Several plants are also used
like “Alecrim”, “Babosa”, “Barba-Timão”, “Calêndula”,
“Cardo-Santo”, “Espinheira-Santa”, “Mil-Folhas” and
“Tanchagem”. These plants, in spite of be used
thoroughly they lack of deeper studies in the clinical
and pharmacotechnics aspects. The use of Brassica
is reported by Balbach and Boarim (1993) that
suggested it to be used for the treatment of several
illnesses as abscesses, hemorrhoids, facial and
dental nevralgy, intestinal disturbances and wounds
in general. These authors (Balbach and Boarim, 1993)
refer to coming information of the year of 1881, when
Dr. Blanc, of the University of Paris published classic
work about the use of the cabbage, entitled “Les
propriétès médicales de la feville de chou “ (The
medicinal properties of the cabbage leaf).
Rev. Bras. Pl. Med., Botucatu, v.8, n.esp., p.136-138, 2006.
137
MATERIAL AND METHOD
FIGURE 1. Evolution of the cicatrization process of wounds
in Cavia cobaya, treated with KollagenaseÒ, ointment of
Brassica sp. and with saline solution (control).
TABLE 1. Rate values of cicatrization calculated for each
treatment, and the coefficient of regression determination
(R2).
It was used nine white adult animals (Cavia
cobaya), of both sexes, weighing around 600g and
purchasing from LARA (Regional Laboratory of Animal
Facilities), Pedro Leopoldo-MG, Brazil. The animals
were feeded with food and water ad libitum. They were
randomly separated in three groups with three
individuals each. Each cage contained an animal and
it was properly identified. The first group received a
plant extract from Brassica sp healing ointment
PhytoderminÒ, the second group received a standard
industrialized healing ointment KolagenaseÒ and the
third, NaCl 0.9% saline solution (control group).
The hair of the lateral right of each animal
was removed with the aid of an electric shaver. The
surgical wounds was induced using aseptic
techniques and each animal received local anesthesia
of lidocain 2% plus fenilefrin 1:1000 in the muscle in
the dose of 0.5 ml approximately. The surgical wounds
had approximately a area of 2.0 cm2. The wounds
were obtained by the removal of the skin and of the
subcutaneous tissue. The borders of the wounds were
measured and the data individually logged. In all
animals the surgical procedure was accomplished by
the same person. The animals received the treatments
twice a day, until the complete cicatrization of the
wounds. The wounds were measured daily.
To the cicatrization curve in function of the
time was adjusted of lineal regression with the
objective of esteeming the mean of the rate
cicatrization for each treatment. The cicatrization rate
was obtained and compared statistically.
RESULT AND DISCUSSION
The present work has for objective to study
the effectiveness of the healing ointment developed
by the handmade pharmacy. This phytotherapic
product (PhytoderminÒ) is obtained starting from the
plant extract of Brassica sp (BrassiskinÒ).
The cicatrization curve of the wounds
submitted to the different treatments can be observed
in the Figure 1. In the monitored animals, the
cicatrization happened around twenty days. The
cicatrization process was slower in the controls
treatment. Based in the curve of cicatrization evolution,
it was possible to estimate the cicatrization rates for
each treatment, with aid of the lineal model (Table 1).
Based on the values of the rate for each
treatment, the comparative test of average (Tukey,
p=0.05) was applied. It was possible to verify that the
TABLE 2. Comparison among the cicatrization rates for the treated wounds with KollagenaseÒ, ointment of Brassica sp
and with saline solution (control).
Obs.: Average proceeded by the same letter doesn’t differ to each other by the Tukey´s test (P>0.05)
Rev. Bras. Pl. Med., Botucatu, v.8, n.esp., p.136-138, 2006.
138
cicatrization rates obtained with use of the ointment
of Brassica sp. (PhytoderminÒ) and, KollagenaseÒ
didn’t differ significantly to each other. Both rates were
larger than the rate observed in the controls treatment
(Table 2), suggesting that the phytotherapeutic product
(Phytoderminâ) used to heal the woods had similar
effect of the commercial industrialized product
(Kollagenaseâ).
ACKNOWLEDGMENT
The authors thank the support of the Fundação de
Amparo à Pesquisa do Estado de Minas Gerais
(FAPEMIG).
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