American Physiological Society International Opportunity Program
“Novel Technologies for the Study of Cardiovascular Biology and Fluid Homeostasis”
Dates: August 13-15th
Venue: School of Dentistry, São Paulo State University/UNESP, Araraquara – SP, Brazil
Organizers
Debora Simões Almeida Colombari
Department of Physiology and Pathology
School of Dentistry
São Paulo State University/UNESP
Araraquara – SP, Brazil
Email: [email protected]
Gina L. C. Yosten
Department of Pharmacological and Physiological Science
Saint Louis University School of Medicine
1402 S. Grand Blvd
Saint Louis, MO 63108
Email: [email protected], [email protected]
O Workshop está vinculado ao Programa Interinstitucional de Pós Graduação em Ciências
Fisiológicas (PIPGCF) – Associação Ampla UFSCar/UNESP e será ministrado dentro do PIPGCF
como disciplina com carga horária de 2 créditos.
13/08: Discovery
0800 Seminar 1—Carol Elias, University of Michigan
“Generation and Utilization of Genetic Models”
0900 Student Symposia 1—Senior Graduate students and Postdocs present their work in 10-15
minute presentations and receive feedback from their peers and from faculty
1015 Coffee Break
1030 Workshop 1—Gina LC Yosten, Saint Louis University
“Practical Application of Bioinformatic Tools”
Hands-on workshop will guide trainees through the practical application of
bioinformatic tools to their research. Topics covered could include designing and
virtually testing primers, designing siRNA constructs, mining microarray and SNP
data, and gene analyses
1200 Lunch
1300 Student Symposia 2—Senior Graduate students and Postdocs present their work in 10-15
minute presentations and receive feedback from their peers and from faculty
1500 Coffee Break
1515 One-on-One Session—Students/Postdocs will sign up for 15-30 minute sessions with
individual faculty to discuss their research and implementation of new tools for their work. Prior
to the Symposia, the faculty will provide a paragraph describing their areas of expertise.
14/08: Methods and Application
0800 Seminar 2—Willis K Samson, Saint Louis University
“From Discovery to Physiology: Role of Small Peptides in Cardiovascular
Physiology”
09:00 – Seminar 2 - Debora S A Colombari, São Paulo State University
“Methods to record and analyzed sympathetic and phrenic nerve activity”
1000 Coffee Break
1015 - Workshop 2—Debora and Eduardo Colombari and Daniel Zoccal – São Paulo State
University
“Modern Methods Lab and Unknown Conference”
Hands-on workshop designed to give trainees first-hand experience with a modern
technology. We will have 2 groups of 10-12 students. While some groups are working on the in
situ preparation the other groups could meet with Dr. Yosten, Dr. Samson and Dr. Elias to discuss
their research.
EXPERIMENT:
We will use the in situ arterially perfused working-heart brainstem preparation to record
sympathetic and phrenic nerve activity and inject “unknow” peptides in the nucleus of the
solitary tract or area postrema. Students will be presented with “unknowns” and must use
their knowledge to predict the pharmacological substance being tested.
1230 Lunch
1400 – 1600 Continuation of Workshop 2—Debora and Eduardo Colombari and Daniel Zoccal
1600 Discussion of the data obtained in the in situ arterially perfused working-heart brainstem
preparation
15/08: Methods and Application
0800 Seminar 3 – Daniela Accorsi, University of São Paulo
“Current methods on cell recording”
0900 – 1200 Workshop 2— Daniela Accorsi
“Modern Methods Lab and Unknown Conference”
Hands-on workshop designed to give trainees first-hand experience with a modern
technology.
PATCH-CLAMP DATA DISCUSSION:
Data obtained previously with Dr. Daniela Accorsi will be discussed. The data will be related to
the effects of “unknown” peptides perfused in the slice preparation to record neuron activity in
the nucleus of the solitary tract. Students will be presented with “unknowns” and must use
their knowledge to predict the pharmacological substance being tested.
1200 Lunch
1300 – 1600 —Additional time for trainees to discuss data obtained with patch-clamp
1600 Closing Remarks