THE DEVELOPMENT AND CHARACTERIZATION OF HYDROQUINONE
LIPOSOMAL
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MOURA-JÚNIOR, G. , ALMEIDA, J.M.T. , GUSMÃO, D.O. , NETO, E.R.O. , SILVA, C.M.V. ,
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CARDOSO, J.C. , ANDRADE, J.E. , LIMA, C.M.
1
UNIVERSIDADE TIRADENTES – Aracaju/SE – Brazil - e –mail : [email protected]
INSTITUTO DE TECNOLOGIA E PESQUISA – Aracaju/SE – Brazil
3
UNIVERSIDADE FEDERAL DE SERGIPE – São Cristóvão/SE – Brazil
2
Keywords: Liposome, hydroquinone, phospholipids
showed that the drug encapsulation was
1. Introduction
The use of liposomes for drug delivery has
presented interesting results in many medicine
fields. Hydroquinone (HDQ) has been shown to
induce reversible hypopigmentation in man and
possible, but the vesicle stability must be
investigated due the high PI. The zeta potential
value of liposome was – 34,4 mV. These results
point to the suitable stability of liposome.
animals and has been used for decades as a skin
lightening
agent.
susceptible
to
However,
oxidation
this
drug
due
to
is
the
photosensitive characteristic limiting its use.
The aim of this work was the development and
characterization
of
liposomal
4. Conclusion
formulation
containing HDQ in order to improve the drug
The formulation of liposome with hydroquinone
present diameter around 20nm, but with high PI
indicating that the extrusion step may improve
the uniformity of the vesicles.
stability.
Acknowledgments
The authors thanks UNIT, FAPITEC/SE and
2. Methods
CNPq.
The liposomes with HDQ were obtained by film
rehydratation method, using 10 mg of HDQ and
300
mg de
Phospholipon
methanol/chloroform
(1:3).
® 85
G
References
in
Entrapment
Barchini, H. P., Leeuwen, V., Lyklema, J. Eletrodynamics
efficiency of liposomes was determined by the
of liposome dispersions; Langmuir , v. 16, p. 8238-8247,
measurements of HDQ using spectrophotometry
UV at 293 nm.
The liposomes were
2000.
Centis, V., Vermette, P. Physico-chemical properties and
characterized using a Dynamic Light Scattering
cytotoxicity
(Malvern Zetasizer Nanoseries) to determine the
containg human hemoglobin; Biointerfaces, v. 65, p. 239-
vesicles size and polidispersivity index (PI).
3. Results
The entrapment efficiency of liposomes was
99.2% of HDQ. The HDQ liposomes showed
diameter around 20 nm and PI 2.8. The results
assessment
of
PEG-modified
liposomes
246, 2008.
GARDONI, B.LK., M.E.O. SATO, R. PONTAROLO, L.
NORONHA, A. Reichert & S.Z. Serafini. Avaliação Clínica
e Morfológica da Ação da Hidroquinona e do Acido Fítico
como Agentes Despigmentantes. Acta Farm. Bonaerense,
23, 2004.