Characterization of 153Sm-EDTMP Nanoradiopharmaceuticals
G. Weissmüller1, B. F.C. Patricio1,2, R. Santos-Oliveira2,
1
Laboratório de Física Biológica – Instituto de Biofísica Carlos Chagas Filho - Universidade
Federal do Rio de Janeiro, Rio de Janeiro, Brasil.
2
Laboratory of Nanoradiopharmaceuticals - Hospital Universitário Clementino Fraga Filho Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Nanoradiopharmaceuticals are the most promising radiopharmaceuticals. In the case of
bone metastases they represent an important advance due to their unique features. The
Samarium-153 lexidronam (153Sm-EDTMP) is effective and well-tolerated treatment for
painful bone metastases because its efficacy, low cost and low toxicity. In this work we
discuss the formation of such nanoradiopharmaceutical and its characterization using
Atomic Force Microscopy (AFM). Nanoparticles were produced according the double
emulsion method. A solution of poly lactic acid and dichloromethane was poured on a
solution of PVA and EDTMP and then emulsified by sonication producing a water/organic
solvent (W/OS) solution. After that, this solution was emulsified with 4 mL of PVA solution
by sonication producing a W/OS/W emulsion. Then the organic solvent was eliminated by
evaporation under vacuum. The particles were recovered by centrifugation and washed
twice with Milli-Q water to remove the excess of PVA. Nanoparticle morphology was
characterized using MFP-3D-BIOTM (Asylum Research). Samples were prepared by
deposition of particles suspension after centrifugation on freshly cleaved mica. Topography
was captured using TappingMode. Images reveal that all nanoparticles have spherical
shape and a size dispersion ranging from 500-200nm. The samples prepared for analyses
on AFM show nanoparticles aggregation with the large ones in the center and the smaller
ones on the edge. In the phase image it is possible to identify inhomogeneities
corresponding to the different polymers that compose the nanoparticle and that they are
dispersed randomly. In conclusion, nanoparticles were successfully formed by this
methodology and the characterization by AFM was established.
Nanoparticles, drug-delivery, nanoradiopharmaceuticals
���������������������������������������������������������������������������
���������������������������������������������������������������������������������
�����������������������������������������������������