Garlet GP – CV abril 2010 update
Curriculum Vitae
GUSTAVO POMPERMAIER GARLET
Osteoimmunology Lab - Department of Biological Sciences
School of Denstistry of Bauru - University of São Paulo (FOB/USP)
Al. Octávio Pinheiro Brisola, 9-75 - CEP 17012-901 - Bauru/SP/Brazil
[email protected]
Current Position
Associated Professor - Department of Biological Sciences
School of Denstistry of Bauru - University of São Paulo (FOB/USP)
DDS - School of Dentistry – Ponta Grossa State University (UEPG) – Ponta Grossa, PR
MSc - School of Medicine of Ribeirão Preto – University of São Paulo (FMRP-USP), Basic and
Applied Immunology; Thesis: Chemokine expression in human and experimental periodontal
diseases
PhD - School of Medicine of Ribeirão Preto – University of São Paulo (FMRP-USP), Basic and
Applied Immunology; Thesis: The role of cytokines in the immunomodulation of Actinobacillus
actinomycetemcomitans-induced periodontal disease
Posdoctoral Training - School of Medicine of Ribeirão Preto – University of São Paulo (FMRPUSP), Department of Biochemistry and Immunology
Posdoctoral Training - Center for Craniofacial and Dental Genetics, Department of Oral
Biology, School of Dental Medicine, University of Pittsburgh
Personal Information
Name: Gustavo Pompermaier Garlet
Date and Place of Birth: 27 April 1977, Ponta Grossa, Paraná, Brazil
Current Position
Associated Professor
Department of Biological Sciences
School of Denstistry of Bauru - University of São Paulo (FOB/USP)
Education
1999 DDS - School of Dentistry – Ponta Grossa State University (UEPG) – Ponta Grossa, PR
2002 MSc - School of Medicine of Ribeirão Preto – University of São Paulo (FMRP-USP), Basic
and Applied Immunology; Thesis: Chemokine expression in human and experimental
periodontal diseases
2005 PhD - School of Medicine of Ribeirão Preto – University of São Paulo (FMRP-USP), Basic
and Applied Immunology; Thesis: The role of cytokines in the immunomodulation of
Actinobacillus actinomycetemcomitans-induced periodontal disease
2006 Posdoctoral Training - School of Medicine of Ribeirão Preto – University of São Paulo
(FMRP-USP), Department of Biochemistry and Immunology
2008 Posdoctoral Training - Center for Craniofacial and Dental Genetics, Department of Oral
Biology, School of Dental Medicine, University of Pittsburgh
Complementary training
2011 RealTimePCR VIA7 training - Applied Biosystems, São Paulo, Brasil.
2007 RealTimePCR Opticon system training - BioRad, São Paulo, Brasil.
2006 Confocal Microscopy – Leica Microsystems & School of Medicine of Ribeirão Preto of
São Paulo University (FMRP/USP), São Paulo, Brasil.
2004 Chemokine Biology - Brazilian Society of Immunology, SBI, São Paulo, Brasil.
2004 RealTimePCR ABI 7000 training - Applied Biosystems, São Paulo, Brasil.
2003 Flow Cytometry - School of Medicine of Ribeirão Preto of São Paulo University
(FMRP/USP), São Paulo, Brasil.
2001 RealTimePCR ABI 5700 training - Applied Biosystems, São Paulo, Brasil.
Awards and Special Recognition
2010 Clinical Impact Award - AAP - Amercican Academy of Periodontology, American
Academy of Periodontology - AAP.
2009 Basic science section best poster – Brazilian Society of Dental Research (SBPqO). - TNFalpha e IL-10 modulam diferencialmente a formação óssea subsequente ( coupled ) à
reabsorção óssea na periodontite experimental; Gennaro G et al., Sociedade Brasileira de
Pesquisa Odontológica.
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2009 Basic science section best poster – Brazilian Society of Dental Research (SBPqO). Expressão de SOCS-1 e SOCS-3 na doença periodontal induzida experimentalmente; Souza JAC
et al., Sociedade Brasileira de Pesquisa Odontológica.
2009 Periodontology section best poster – Brazilian Society of Dental Research (SBPqO). diferencial de fatores genéticos, microbianos e inflamatórios com os níveis de MMP-1 no
periodonto saudável e com periodontite; Silveira EMV et al., Sociedade Brasileira de Pesquisa
Odontológica.
2009 Research Prize – high impact factor article published - Comissao de Pesquisa FOB/USP Trabalho publicado no Periódico com mais elevado Fator de Impacto (Journal of Leukocyte
Biology) -, Comissao de Pesquisa FOB/USP.
2008 Honorable Mention - Clinical Immunology: BROAD EFFECTS OF THE FUNCTIONAL IL-10
PROMOTER -592 POLYMORPHISM: MODULATION OF IL-10, TIMP-3 AND OPG EXPRESSION
AND ITS ASSOCIATION WITH PERIODONTITIS OUTCOME, Brazilian Society for ImmunologyIMMUNO RIB 2008 XXXIII.
2007 Myaki Issao Prize - Sessão C - Expressão de SOCS-3 e TTp na doença periodontal induzida
experimentalmente. SOUZA JAC et al., Sociedade Brasileira de Pesquisa Odontológica.
2006 Periodontology section best poster – Brazilian Society of Dental Research (SBPqO).
Expression of SOCS (suppressor of cytokine signaling) in diseased periodontal tissues: a stop
signal for disease progression?
2006 Periodontology section best presentation – FOB/USP research meeting – Noninflammatory destructive periodontal disease.
2005 Periodontology section best poster – Brazilian Society of Dental Research (SBPqO).
The dual role of p55 TNF-receptor in Actinobacillus actinomycetemcomitans-induced
experimental periodontitis: host protection and tissue destruction
2005 Orthodontics section best poster – Brazilian Society of Dentistry – ABO-PR/CIOPAR. Cytokine pattern determines the tissue response to orthodontic force through the
establishment of distinct microenvironments in compression and tension sides.
2004 Periodontology section best poster – Brazilian Society of Dental Research (SBPqO).
Cytokine regulation of matrix metalloproteinases, their physiological inhibitors and osteoclast
factors in periodontal diseases.
2004 Pathology section best poster – Brazilian Society of Dental Research (SBPqO).
Chemokine and chemokine receptors expression in human periapical lesions
2003 Edward Hatton – IADR/SBPqO - IFN-g, IL-10 and IL-4 in the immunopathogenesis of
PD: role in determination of disease severity and in the control of infection
2002 Scientific forum best presentation – Brazilian Society of Dentistry - ABO/EAP/PG-PR.
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The role of chemoattractants in the cell migration process in periodontal diseases
Societies
2000 – current: Brazilian Society of Immunology (SBI)
2000 – current: Brazilian Society of Dental Research (SBPqO)
2006 – current: Society for Leukocyte Biology (SLB)
2006 – current: American Society for Bone and Mineral Research (ASBMR)
Research Projects
2009 – current: Osteoclast co-stimulatory molecules in oral osteolytic lesions
Function: PI
2009 – current: The role of cytokines in alveolar bone repair under homeostatic and
infectious/inflammatory conditions
Function: PI
2007 – current: Immunogentics of periodontal diseases: correlations between host genotype
and in situ expression of cytokines, proteases and osteoclastogenic factors
Function: PI
2006 – current: The role of cytokines in bone remodeling – from alveolar bone repair to
orthodontic tooth movement.
Function: PI
2006 – current: The role of Th17 and Regulatory T cells in the immunomodulation of
periodontal diseases
Function: PI
2004 – current: The potential role of CD4+CD25+ regulatory T cells in the immunomodulation
of periodontal diseases
Function: Post-doctoral fellow - Main reseacher; Supervisor: João Santana da Silva
2004 – current: Evaluation of mechanisms of cell migration and bone resorption in periapical
lesions
Function: Coloborator – Head: Fernando Queiroz Cunha (FMRP/USP)
2004 – current: Periodontal Disease and HTLV-1 infection
Function: Coloborator – Head: Edgar Marcelino de Carvalho (UFBA)
2002 – 2005: The role of cytokines in the immunomodulation of human and experimental
periodontal diseases – control of periodontal infection and determination of disease severity
Function: PhD student - main reseacher; Supervisor: João Santana da Silva
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2002 – 2005: Effects of b-FGF and TGF-b in the proliferation and gene expression of human
periodontal ligament cells
Function: Coloborator – Head: Carlos Rossa Jr (FOAr/UNESP – University of Michigan)
2000 – 2002: Chemokine expression in human and experimental periodontal diseases
Function: MSc student - main reseacher; Supervisor: João Santana da Silva
Research and scholarships grants
2010 – 2012 Research grant FAPESP#10/15755-9
2009 – 2011 Research grant CNPq #471616/2009-5
2008 – 2010 Research grant FAPESP#08/03047-0
2007 – 2009 Research grant CNPq #470357/2007-0
2006 – 2010 Research grant Project 1 PRP/USP #ref06/00534-1
2006 – 2010 Research grant FAPESP#06/00534-1
2005 – 2006 Post-doctoral scholarship FAPESP #04/10102-6
2004 – 2005 Research grant FAPESP#04/06731-8
2002 – 2005 PhD scholarship FAPESP #01/09998-7
2000 – 2002 MSc scholarship CNPQ #130274/2000-3
Selected Publications (Peer-reviewed Research Articles)
see complete list at http://lattes.cnpq.br/0901521368129412
2: Andrade Filho PA, Letra A, Cramer A, Prasad JL, Garlet GP, Vieira AR, Ferris
RL, Menezes R. Insights From Studies With Oral Cleft Genes Suggest Associations
between WNT-pathway Genes and Risk of Oral Cancer. J Dent Res. 2011 Mar 10. [Epub
ahead of print] PubMed PMID: 21393552.
5: Ferreira SB Jr, Repeke CE, Raimundo FM, Nunes IS, Avila-Campos MJ, Ferreira
BR, da Silva JS, Campanelli AP, Garlet GP. CCR5 Mediates Pro-osteoclastic and
Osteoclastogenic Leukocyte Chemoattraction. J Dent Res. 2011 Jan 18. [Epub ahead
of print] PubMed PMID: 21245464.
6: Braga SM, Taddei SR, Andrade I Jr, Queiroz-Junior CM, Garlet GP, Repeke CE,
Teixeira MM, da Silva TA. Effect of diabetes on orthodontic tooth movement in a
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mouse model. Eur J Oral Sci. 2011 Feb;119(1):7-14. doi:
10.1111/j.1600-0722.2010.00793.x. PubMed PMID: 21244505.
7: da Fonseca DM, Trombone AP, Repeke CE, Avila-Campos MJ, Coelho-Castelo AA,
Silva JS, Campanelli AP, Deperon Bonato VL, Garlet GP. Functional interferences
in host inflammatory immune response by airway allergic inflammation restrain
experimental periodontitis development in mice. J Clin Periodontol. 2011
Feb;38(2):131-41. doi: 10.1111/j.1600-051X.2010.01660.x. Epub 2010 Dec 9. PubMed
PMID: 21143491.
10: Garlet GP. Destructive and protective roles of cytokines in periodontitis: a
re-appraisal from host defense and tissue destruction viewpoints. J Dent Res.
2010 Dec;89(12):1349-63. Epub 2010 Aug 25. Review. PubMed PMID: 20739705.
11: Garlet GP, Cardoso CR, Mariano FS, Claudino M, de Assis GF, Campanelli AP,
Avila-Campos MJ, Silva JS. Regulatory T cells attenuate experimental
periodontitis progression in mice. J Clin Periodontol. 2010 Jul;37(7):591-600.
PubMed PMID: 20642629.
14: Trombone AP, Claudino M, Colavite P, de Assis GF, Avila-Campos MJ, Silva JS,
Campanelli AP, Ibañez OM, De Franco M, Garlet GP. Periodontitis and arthritis
interaction in mice involves a shared hyper-inflammatory genotype and functional
immunological interferences. Genes Immun. 2010 Sep;11(6):479-89. Epub 2010 Apr
29. PubMed PMID: 20428191.
15: Carvalho FM, Tinoco EM, Deeley K, Duarte PM, Faveri M, Marques MR, Mendonça
AC, Wang X, Cuenco K, Menezes R, Garlet GP, Vieira AR. FAM5C contributes to
aggressive periodontitis. PLoS One. 2010 Apr 7;5(4):e10053. PubMed PMID:
20383335; PubMed Central PMCID: PMC2850931.
16: Claudino M, Garlet TP, Cardoso CR, de Assis GF, Taga R, Cunha FQ, Silva JS,
Garlet GP. Down-regulation of expression of osteoblast and osteocyte markers in
periodontal tissues associated with the spontaneous alveolar bone loss of
interleukin-10 knockout mice. Eur J Oral Sci. 2010 Feb;118(1):19-28. PubMed PMID:
20156261.
19: Garlet TP, Fukada SY, Saconato IF, Avila-Campos MJ, da Silva TA, Garlet GP,
Cunha Fde Q. CCR2 deficiency results in increased osteolysis in experimental
periapical lesions in mice. J Endod. 2010 Feb;36(2):244-50. Epub 2009 Oct 23.
PubMed PMID: 20113782.
20: Repeke CE, Ferreira SB Jr, Claudino M, Silveira EM, de Assis GF, Avila-Campos
MJ, Silva JS, Garlet GP. Evidences of the cooperative role of the chemokines
CCL3, CCL4 and CCL5 and its receptors CCR1+ and CCR5+ in RANKL+ cell migration
throughout experimental periodontitis in mice. Bone. 2010 Apr;46(4):1122-30. Epub
2010 Jan 4. PubMed PMID: 20053385.
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21: Garlet GP, Giozza SP, Silveira EM, Claudino M, Santos SB, Avila-Campos MJ,
Martins W Jr, Cardoso CR, Trombone AP, Campanelli AP, Carvalho EM, Silva JS.
Association of human T lymphotropic virus 1 amplification of periodontitis
severity with altered cytokine expression in response to a standard
periodontopathogen infection. Clin Infect Dis. 2010 Feb 1;50(3):e11-8. PubMed
PMID: 20038241.
25: Andrade I Jr, Taddei SR, Garlet GP, Garlet TP, Teixeira AL, Silva TA,
Teixeira MM. CCR5 down-regulates osteoclast function in orthodontic tooth
movement. J Dent Res. 2009 Nov;88(11):1037-41. PubMed PMID: 19828893.
26: Repeke CE, Trombone AP, Ferreira SB Jr, Cardoso CR, Silveira EM, Martins W
Jr, Trevilatto PC, Silva JS, Campanelli AP, Garlet GP. Strong and persistent
microbial and inflammatory stimuli overcome the genetic predisposition to higher
matrix metalloproteinase-1 (MMP-1) expression: a mechanistic explanation for the
lack of association of MMP1-1607 single-nucleotide polymorphism genotypes with
MMP-1 expression in chronic periodontitis lesions. J Clin Periodontol. 2009
Sep;36(9):726-38. Epub 2009 Jul 29. PubMed PMID: 19659894.
31: Cardoso CR, Garlet GP, Crippa GE, Rosa AL, Júnior WM, Rossi MA, Silva JS.
Evidence of the presence of T helper type 17 cells in chronic lesions of human
periodontal disease. Oral Microbiol Immunol. 2009 Feb;24(1):1-6. PubMed PMID:
19121062.
32: Trombone AP, Cardoso CR, Repeke CE, Ferreira SB Jr, Martins W Jr, Campanelli
AP, Avila-Campos MJ, Trevilatto PC, Silva JS, Garlet GP. Tumor necrosis
factor-alpha -308G/A single nucleotide polymorphism and red-complex
periodontopathogens are independently associated with increased levels of tumor
necrosis factor-alpha in diseased periodontal tissues. J Periodontal Res. 2009
Oct;44(5):598-608. Epub 2008 Dec 11. PubMed PMID: 19076989.
33: Fukada SY, Silva TA, Saconato IF, Garlet GP, Avila-Campos MJ, Silva JS, Cunha
FQ. iNOS-derived nitric oxide modulates infection-stimulated bone loss. J Dent
Res. 2008 Dec;87(12):1155-9. PubMed PMID: 19029085.
34: Menezes R, Garlet TP, Trombone AP, Repeke CE, Letra A, Granjeiro JM,
Campanelli AP, Garlet GP. The potential role of suppressors of cytokine signaling
in the attenuation of inflammatory reaction and alveolar bone loss associated
with apical periodontitis. J Endod. 2008 Dec;34(12):1480-4. Epub 2008 Oct 18.
PubMed PMID: 19026878; PubMed Central PMCID: PMC2719713.
35: Trombone AP, Ferreira SB Jr, Raimundo FM, de Moura KC, Avila-Campos MJ, Silva
JS, Campanelli AP, De Franco M, Garlet GP. Experimental periodontitis in mice
selected for maximal or minimal inflammatory reactions: increased inflammatory
immune responsiveness drives increased alveolar bone loss without enhancing the
control of periodontal infection. J Periodontal Res. 2009 Aug;44(4):443-51. Epub
2008 Oct 7. PubMed PMID: 18973535.
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37: Claudino M, Trombone AP, Cardoso CR, Ferreira SB Jr, Martins W Jr, Assis GF,
Santos CF, Trevilatto PC, Campanelli AP, Silva JS, Garlet GP. The broad effects
of the functional IL-10 promoter-592 polymorphism: modulation of IL-10, TIMP-3,
and OPG expression and their association with periodontal disease outcome. J
Leukoc Biol. 2008 Dec;84(6):1565-73. Epub 2008 Aug 25. PubMed PMID: 18725394.
38: Menezes R, Garlet TP, Letra A, Bramante CM, Campanelli AP, Figueira Rde C,
Sogayar MC, Granjeiro JM, Garlet GP. Differential patterns of receptor activator
of nuclear factor kappa B ligand/osteoprotegerin expression in human periapical
granulomas: possible association with progressive or stable nature of the
lesions. J Endod. 2008 Aug;34(8):932-8. PubMed PMID: 18634923; PubMed Central
PMCID: PMC2719712.
39: Ferreira SB Jr, Trombone AP, Repeke CE, Cardoso CR, Martins W Jr, Santos CF,
Trevilatto PC, Avila-Campos MJ, Campanelli AP, Silva JS, Garlet GP. An
interleukin-1beta (IL-1beta) single-nucleotide polymorphism at position 3954 and
red complex periodontopathogens independently and additively modulate the levels
of IL-1beta in diseased periodontal tissues. Infect Immun. 2008
Aug;76(8):3725-34. Epub 2008 Jun 9. PubMed PMID: 18541658; PubMed Central PMCID:
PMC2493201.
40: Cardoso CR, Garlet GP, Moreira AP, Júnior WM, Rossi MA, Silva JS.
Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory
infiltrate of human chronic periodontitis. J Leukoc Biol. 2008 Jul;84(1):311-8.
Epub 2008 May 1. PubMed PMID: 18451325.
41: Garlet TP, Coelho U, Repeke CE, Silva JS, Cunha Fde Q, Garlet GP.
Differential expression of osteoblast and osteoclast chemmoatractants in
compression and tension sides during orthodontic movement. Cytokine. 2008
Jun;42(3):330-5. Epub 2008 Apr 11. PubMed PMID: 18406624.
42: Garlet GP, Cardoso CR, Campanelli AP, Garlet TP, Avila-Campos MJ, Cunha FQ,
Silva JS. The essential role of IFN-gamma in the control of lethal
Aggregatibacter actinomycetemcomitans infection in mice. Microbes Infect. 2008
Apr;10(5):489-96. Epub 2008 Jan 25. PubMed PMID: 18403243.
45: Garlet TP, Coelho U, Silva JS, Garlet GP. Cytokine expression pattern in
compression and tension sides of the periodontal ligament during orthodontic
tooth movement in humans. Eur J Oral Sci. 2007 Oct;115(5):355-62. PubMed PMID:
17850423.
47: Silva TA, Garlet GP, Fukada SY, Silva JS, Cunha FQ. Chemokines in oral
inflammatory diseases: apical periodontitis and periodontal disease. J Dent Res.
2007 Apr;86(4):306-19. Review. PubMed PMID: 17384024.
48: Garlet GP, Cardoso CR, Campanelli AP, Ferreira BR, Avila-Campos MJ, Cunha FQ,
Silva JS. The dual role of p55 tumour necrosis factor-alpha receptor in
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Actinobacillus actinomycetemcomitans-induced experimental periodontitis: host
protection and tissue destruction. Clin Exp Immunol. 2007 Jan;147(1):128-38.
PubMed PMID: 17177972; PubMed Central PMCID: PMC1810448.
49: Garlet GP, Cardoso CR, Campanelli AP, Martins W Jr, Silva JS. Expression of
suppressors of cytokine signaling in diseased periodontal tissues: a stop signal
for disease progression? J Periodontal Res. 2006 Dec;41(6):580-4. PubMed PMID:
17076785.
50: Garlet GP, Cardoso CR, Silva TA, Ferreira BR, Avila-Campos MJ, Cunha FQ,
Silva JS. Cytokine pattern determines the progression of experimental periodontal
disease induced by Actinobacillus actinomycetemcomitans through the modulation of
MMPs, RANKL, and their physiological inhibitors. Oral Microbiol Immunol. 2006
Feb;21(1):12-20. PubMed PMID: 16390336.
52: Garlet GP, Avila-Campos MJ, Milanezi CM, Ferreira BR, Silva JS.
Actinobacillus actinomycetemcomitans-induced periodontal disease in mice:
patterns of cytokine, chemokine, and chemokine receptor expression and leukocyte
migration. Microbes Infect. 2005 Apr;7(4):738-47. Epub 2005 Mar 22. PubMed PMID:
15850760.
54: Garlet GP, Martins W Jr, Fonseca BA, Ferreira BR, Silva JS. Matrix
metalloproteinases, their physiological inhibitors and osteoclast factors are
differentially regulated by the cytokine profile in human periodontal disease. J
Clin Periodontol. 2004 Aug;31(8):671-9. PubMed PMID: 15257746.
55: Garlet GP, Martins W Jr, Ferreira BR, Milanezi CM, Silva JS. Patterns of
chemokines and chemokine receptors expression in different forms of human
periodontal disease. J Periodontal Res. 2003 Apr;38(2):210-7. PubMed PMID:
12608917.
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