57º Congresso Brasileiro de Genética
Resumos do 57º Congresso Brasileiro de Genética • 30 de agosto a 2 de setembro de 2011
Centro de Convenções do Hotel Monte Real Resort • Águas de Lindóia • SP • Brasil
www.sbg.org.br - ISBN 978-85-89109-06-2
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In vivo modulatory effect of orange juice
evaluated by the comet assay
Mendonça, VRR; Pereira, H; Moreira, AC; Porto, TF; Acésio, NO; Cecchi, AO.
Universidade de Franca – UNIFRAN, Franca/SP
[email protected]
Keywords: Orange juice, Comet assay, Blood cells
Deficiencies of vitamins and minerals are related to genomic instability, which can cause an increase in breaks and
oxidative DNA lesions. Orange juice has a preventive action attributed to some vitamins and other compounds such
as phenolic compounds. World consumption of orange juice has increased and consequentely resulted in increased
industrial production in many countries, including Brazil, which accounts for 70% of industries. This study aimed to
investigate the potential antigenotoxic of orange juice (fresh and after 3 and 5 hours of preparation) in mice treated
with the mutagen cyclophosphamide (CPA). Swiss male mice were divided in eight groups of treatments, including the
negative control. Fresh juice (immediately after preparation) and with 3 and 5 h after preparation, kept at room temperature without light protection, was administered by gavage (10 mL/kg body weight, b.w.), and CPA (100 mg/kg b.w.),
intraperitoneally. The antigenotoxicity effects of orange juice on DNA damage in mice peripheral blood were studied
using alkaline single cell gel electrophoresis. The blood samples was collected 24 h after the treatments. The Comet assay revealed that orange juice did not show potential to induce DNA damage, independent of time-preparation. When
antigenotoxic activity was analyzed, the results showed that the orange juice decreased the rate of DNA damage in relation to the positive control (CPA). In conclusion, consumption of orange juice can contribute to DNA protection.
Financial Support: University of Franca